Human NT ELISA Kit

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  • Alternative name

    Nitrotyrosine ELISA Kit,NT ELISA Kit,3-Nitrotyrosine ELISA Kit,3-Nitro-L-tyrosine ELISA Kit,3NT ELISA Kit

  • Catalog
  • species
  • GeneNT
  • Other Species Human NT BNP ELISA KitHuman NT proBNP ELISA KitHuman NT-ProANP ELISA KitHuman NT-ProBNP ELISA KitHuman NT-ProCNP ELISA KitHuman NT ELISA KitHuman NT 1 ELISA KitHuman NT 2 ELISA KitHuman NT 3 ELISA KitHuman NT 4 ELISA KitHuman NT-5 ELISA KitHuman NT 2R ELISA KitHuman NT-2R ELISA KitHuman NT-1 ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity4.2 pg/mL
  • Intended UseHuman NT ELISA Kit allows for the in vitro quantitative determination of NT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    Human NT ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to NT, During the reaction,NT in the sample or standard competes with a fixed amount of biotin-labeled NT for sites on a pre-coated Monoclonal antibody specific to NT. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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