Human NPFFR2 ELISA Kit

Alternative name
Human G-protein coupled receptor 74 ELISA Kit;Human G-protein coupled receptor HLWAR77 ELISA Kit;Human neuropeptide G-protein coupled receptor ELISA Kit;Human GPR74 ELISA Kit;Human NPFF2 ELISA Kit;Human NPGPR ELISA Kit;Human HLWAR77 ELISA Kit;Human neuropeptide FF receptor 2 ELISA Kit;Human neuropeptide FF 2 ELISA Kit;
Catalog
E026298
species
Human
GeneNPFFR2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.375ng/ml
Intended UseHuman NPFFR2 ELISA Kit allows for the in vitro quantitative determination of NPFFR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPFFR2. During the reaction,NPFFR2 in the sample or standard competes with a fixed amount of biotin-labeled NPFFR2 for sites on a pre-coated Monoclonal antibody specific to NPFFR2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NPFFR2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Neuropeptide FF receptor 2 Protein information
Uniprot ID NPFF2_HUMAN
Uniprot AC Q9Y5X5; Q96RV1; Q9NR49;
UniGene Hs.99231;
GeneID 10886
KEGG hsa:10886;
Human Neuropeptide FF receptor 2 Protein SEQUENCE
SEQUENCE 522 AA; 60270 MW; 40CB9FCD42F77041 CRC64;
MNSFFGTPAA SWCLLESDVS SAPDKEAGRE RRALSVQQRG GPAWSGSLEW
SRQSAGDRRR LGLSRQTAKS SWSRSRDRTC CCRRAWWILV PAADRARRER
FIMNEKWDTN SSENWHPIWN VNDTKHHLYS DINITYVNYY LHQPQVAAIF
IISYFLIFFL CMMGNTVVCF IVMRNKHMHT VTNLFILNLA ISDLLVGIFC
MPITLLDNII AGWPFGNTMC KISGLVQGIS VAASVFTLVA IAVDRFQCVV
YPFKPKLTIK TAFVIIMIIW VLAITIMSPS AVMLHVQEEK YYRVRLNSQN
KTSPVYWCRE DWPNQEMRKI YTTVLFANIY LAPLSLIVIM YGRIGISLFR
AAVPHTGRKN QEQWHVVSRK KQKIIKMLLI VALLFILSWL PLWTLMMLSD
YADLSPNELQ IINIYIYPFA HWLAFGNSSV NPIIYGFFNE NFRRGFQEAF
QLQLCQKRAK PMEAYALKAK SHVLINTSNQ LVQESTFQNP HGETLLYRKS
AEKPQQELVM EELKETTNSS EI
UCSC uc003hgg.3; human. [Q9Y5X5-1];

Category

Human NPFFR2 ELISA Kit