Human NDNF ELISA Kit

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  • Alternative name

    Human Neuron-derived neurotrophic factor ELISA Kit;Human C4orf31 ELISA Kit;Human NORD ELISA Kit;Human neuron derived neurotrophic factor ELISA Kit;Human protein NDNF ELISA Kit;Human fibronectin type-III domain-containing protein C4orf31 ELISA Kit;

  • Catalog
    E026242
  • species
    Human
  • GeneNDNF
  • Standard CurveHuman NDNF ELISA Kit
  • Other Species Mouse Ndnf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuron Derived Neurotrophic Factor (NDNF). No significant cross-reactivity or interference between Neuron Derived Neurotrophic Factor (NDNF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.4pg/mL.
  • Intended UseHuman NDNF ELISA Kit allows for the in vitro quantitative determination of NDNF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuron Derived Neurotrophic Factor (NDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuron Derived Neurotrophic Factor (NDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuron Derived Neurotrophic Factor (NDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuron Derived Neurotrophic Factor (NDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein NDNF Protein information
  • Uniprot ID NDNF_HUMAN
  • Uniprot AC Q8TB73; A8K0Q0; Q6UWE5; Q9H5P7;
  • UniGene Hs.709520;
  • GeneID 79625
  • KEGG hsa:79625;
  • Human Protein NDNF Protein SEQUENCE
  • SEQUENCE 568 AA; 64673 MW; 140498575659675F CRC64;

    MVLLHWCLLW LLFPLSSRTQ KLPTRDEELF QMQIRDKAFF HDSSVIPDGA

    EISSYLFRDT PKRYFFVVEE DNTPLSVTVT PCDAPLEWKL SLQELPEDRS

    GEGSGDLEPL EQQKQQIINE EGTELFSYKG NDVEYFISSS SPSGLYQLDL

    LSTEKDTHFK VYATTTPESD QPYPELPYDP RVDVTSLGRT TVTLAWKPSP

    TASLLKQPIQ YCVVINKEHN FKSLCAVEAK LSADDAFMMA PKPGLDFSPF

    DFAHFGFPSD NSGKERSFQA KPSPKLGRHV YSRPKVDIQK ICIGNKNIFT

    VSDLKPDTQY YFDVFVVNIN SNMSTAYVGT FARTKEEAKQ KTVELKDGKI

    TDVFVKRKGA KFLRFAPVSS HQKVTFFIHS CLDAVQIQVR RDGKLLLSQN

    VEGIQQFQLR GKPKAKYLVR LKGNKKGASM LKILATTRPT KQSFPSLPED

    TRIKAFDKLR TCSSATVAWL GTQERNKFCI YKKEVDDNYN EDQKKREQNQ

    CLGPDIRKKS EKVLCKYFHS QNLQKAVTTE TIKGLQPGKS YLLDVYVIGH

    GGHSVKYQSK VVKTRKFC

  • UCSC uc003idq.2; human.;


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