SpecificityThis assay has high sensitivity and excellent specificity for detection of NMU. No significant cross-reactivity or interference between NMU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NMU and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman NMU ELISA Kit allows for the in vitro quantitative determination of NMU , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: NMU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NMU antibody and an NMU-HRP conjugate. The assay sample and buffer are incubated together with NMU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NMU concentration since NMU from samples and NMU-HRP conjugate compete for the anti-NMU antibody binding site. Since the number of sites is limited, as more sites are occupied by NMU from the sample, fewer sites are left to bind NMU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NMU concentration in each sample is interpolated from this standard curve.