Human NMU ELISA Kit

Alternative name
Human NmU-25 ELISA Kit;Human neuromedin U ELISA Kit;Human neuromedin-U ELISA Kit;Human prepro-NMU ELISA Kit;
Catalog
E026228
species
Human
GeneNMU
Standard Curve
Other Species Mouse Nmu ELISA Kit Canine NMU ELISA Kit Guinea Pig NMU ELISA Kit Chicken NMU ELISA Kit Porcine NMU ELISA Kit Rabbit NMU ELISA Kit Rat Nmu ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of NMU. No significant cross-reactivity or interference between NMU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NMU and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman NMU ELISA Kit allows for the in vitro quantitative determination of NMU , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalPrinciple of the assay: NMU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NMU antibody and an NMU-HRP conjugate. The assay sample and buffer are incubated together with NMU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NMU concentration since NMU from samples and NMU-HRP conjugate compete for the anti-NMU antibody binding site. Since the number of sites is limited, as more sites are occupied by NMU from the sample, fewer sites are left to bind NMU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NMU concentration in each sample is interpolated from this standard curve.
Human Neuromedin-U-25 Protein information
Uniprot ID NMU_HUMAN
Uniprot AC P48645;
UniGene Hs.418367;
GeneID 10874
KEGG hsa:10874;
Human Neuromedin-U-25 Protein SEQUENCE
SEQUENCE 174 AA; 19741 MW; 8ABD8138769FA815 CRC64;
MLRTESCRPR SPAGQVAAAS PLLLLLLLLA WCAGACRGAP ILPQGLQPEQ
QLQLWNEIDD TCSSFLSIDS QPQASNALEE LCFMIMGMLP KPQEQDEKDN
TKRFLFHYSK TQKLGKSNVV SSVVHPLLQL VPHLHERRMK RFRVDEEFQS
PFASQSRGYF LFRPRNGRRS AGFI
UCSC uc003hbc.3; human.;

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Human NMU ELISA Kit

Human NmU-25 ELISA Kit;Human neuromedin U ELISA Kit;Human neuromedin-U ELISA Kit;Human prepro-NMU ELISA Kit;