Human NMU ELISA Kit

Alternative name
Human NmU-25 ELISA Kit;Human neuromedin U ELISA Kit;Human neuromedin-U ELISA Kit;Human prepro-NMU ELISA Kit;
Catalog
E026228
species
Human
GeneNMU
SpecificityThis assay has high sensitivity and excellent specificity for detection of NMU. No significant cross-reactivity or interference between NMU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NMU and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman NMU ELISA Kit allows for the in vitro quantitative determination of NMU , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalPrinciple of the assay: NMU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NMU antibody and an NMU-HRP conjugate. The assay sample and buffer are incubated together with NMU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NMU concentration since NMU from samples and NMU-HRP conjugate compete for the anti-NMU antibody binding site. Since the number of sites is limited, as more sites are occupied by NMU from the sample, fewer sites are left to bind NMU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NMU concentration in each sample is interpolated from this standard curve.
Human Neuromedin-U-25 Protein information
Uniprot ID NMU_HUMAN
Uniprot AC P48645;
UniGene Hs.418367;
GeneID 10874
KEGG hsa:10874;
Human Neuromedin-U-25 Protein SEQUENCE
SEQUENCE 174 AA; 19741 MW; 8ABD8138769FA815 CRC64;
MLRTESCRPR SPAGQVAAAS PLLLLLLLLA WCAGACRGAP ILPQGLQPEQ
QLQLWNEIDD TCSSFLSIDS QPQASNALEE LCFMIMGMLP KPQEQDEKDN
TKRFLFHYSK TQKLGKSNVV SSVVHPLLQL VPHLHERRMK RFRVDEEFQS
PFASQSRGYF LFRPRNGRRS AGFI
UCSC uc003hbc.3; human.;

Human NMU ELISA Kit

Human NmU-25 ELISA Kit;Human neuromedin U ELISA Kit;Human neuromedin-U ELISA Kit;Human prepro-NMU ELISA Kit;

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