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Human NMB ELISA Kit

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  • Alternative name

    Human neuromedin B ELISA Kit;Human neuromedin-B ELISA Kit;Human Neuromedin-B-32 ELISA Kit;Human neuromedin-beta ELISA Kit;
  • Catalog
    E026222
  • species
    Human
  • GeneNMB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuromedin B (NMB). No significant cross-reactivity or interference between Neuromedin B (NMB) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 43.5pg/mL.
  • Intended UseHuman NMB ELISA Kit allows for the in vitro quantitative determination of NMB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuromedin B (NMB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuromedin B (NMB) and unlabeled Neuromedin B (NMB) (Standards or samples) with the pre-coated antibody specific to Neuromedin B (NMB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuromedin B (NMB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuromedin B (NMB) in the sample.
  • Human Neuromedin-B Protein information
  • Uniprot ID NMB_HUMAN
  • Uniprot AC P08949; Q96A06; Q96HH5;
  • UniGene Hs.386470;
  • GeneID 4828
  • KEGG hsa:4828;
  • Human Neuromedin-B Protein SEQUENCE

  • SEQUENCE 121 AA; 13252 MW; 10E16BA4886F03FB CRC64;

    MARRAGGARM FGSLLLFALL AAGVAPLSWD LPEPRSRASK IRVHSRGNLW

    ATGHFMGKKS LEPSSPSPLG TAPHTSLRDQ RLQLSHDLLG ILLLKKALGV

    SLSRPAPQIQ YRRLLVQILQ K

  • UCSC uc002bkz.4; human. [P08949-1];



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