SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuromedin B (NMB).
No significant cross-reactivity or interference between Neuromedin B (NMB) and analogues was observed.
Intended UseHuman NMB ELISA Kit allows for the in vitro quantitative determination of NMB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuromedin B (NMB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuromedin B (NMB) and unlabeled Neuromedin B (NMB) (Standards or samples) with the pre-coated antibody specific to Neuromedin B (NMB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuromedin B (NMB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuromedin B (NMB) in the sample.