Human BIRC7 ELISA Kit

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  • Alternative name

    Human kidney inhibitor of apoptosis protein ELISA Kit;Human KIAP ELISA Kit;Human LIVIN ELISA Kit;Human melanoma inhibitor of apoptosis protein ELISA Kit;Human ML-IAP ELISA Kit;Human RING finger protein 50 ELISA Kit;Human RING-type E3 ubiquitin transferase BIRC7 ELISA Kit;Human Truncated livin ELISA Kit;Human p30-Livin ELISA Kit;Human tLivin ELISA Kit;Human MLIAP ELISA Kit;Human RNF50 ELISA Kit;Human baculoviral IAP repeat containing 7 ELISA Kit;Human baculoviral IAP repeat-containing protein 7 ELISA Kit;Human RING-type E3 ubiquitin transferase BIRC7 ELISA Kit;Human livin inhibitor of apoptosis ELISA Kit;

  • Catalog
    E002622
  • species
    Human
  • GeneBIRC7
  • Standard CurveHuman BIRC7 ELISA Kit
  • Other Species Mouse Birc7 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman BIRC7 ELISA Kit allows for the in vitro quantitative determination of BIRC7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: Anti-livin ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti-livin antigen (Anti-livin) and an Anti-livin-HRP conjugate. The assay sample and buffer are incubated together with Anti-livin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-livin concentration since Anti-livin from samples and Anti-livin-HRP conjugate compete for the Anti-livin antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-livin from the sample, fewer sites are left to bind Anti-livin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-livin concentration in each sample is interpolated from this standard curve
  • Human Baculoviral IAP repeat-containing protein 7 30kDa subunit Protein information
  • Uniprot ID BIRC7_HUMAN
  • Uniprot AC Q96CA5; Q9BQV0; Q9H2A8; Q9HAP7;
  • UniGene Hs.256126;
  • GeneID 79444
  • KEGG hsa:79444;
  • Human Baculoviral IAP repeat-containing protein 7 30kDa subunit Protein SEQUENCE
  • SEQUENCE 298 AA; 32798 MW; B2EAAEE531BEC101 CRC64;

    MGPKDSAKCL HRGPQPSHWA AGDGPTQERC GPRSLGSPVL GLDTCRAWDH

    VDGQILGQLR PLTEEEEEEG AGATLSRGPA FPGMGSEELR LASFYDWPLT

    AEVPPELLAA AGFFHTGHQD KVRCFFCYGG LQSWKRGDDP WTEHAKWFPS

    CQFLLRSKGR DFVHSVQETH SQLLGSWDPW EEPEDAAPVA PSVPASGYPE

    LPTPRREVQS ESAQEPGGVS PAEAQRAWWV LEPPGARDVE AQLRRLQEER

    TCKVCLDRAV SIVFVPCGHL VCAECAPGLQ LCPICRAPVR SRVRTFLS

  • UCSC uc002yei.5; human. [Q96CA5-1];



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