SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurokinin B (NKB).
No significant cross-reactivity or interference between Neurokinin B (NKB) and analogues was observed.
Intended UseHuman NKB ELISA Kit allows for the in vitro quantitative determination of NKB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurokinin B (NKB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurokinin B (NKB) and unlabeled Neurokinin B (NKB) (Standards or samples) with the pre-coated antibody specific to Neurokinin B (NKB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurokinin B (NKB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurokinin B (NKB) in the sample.