Human ng ELISA Kit;Human RC3 ELISA Kit;Human hng ELISA Kit;Human neurogranin ELISA Kit;Human calmodulin-binding protein ELISA Kit;Human neurogranin (protein kinase C substrate, RC3) ELISA Kit;Human protein kinase C substrate ELISA Kit;
Catalog
E026195
species
Human
GeneNRGN
SpecificityThis assay has high sensitivity and excellent specificity for detection of NRGN . No significant cross-reactivity or interference between NRGN and analogues was observed.
Intended UseHuman NRGN ELISA Kit allows for the in vitro quantitative determination of NRGN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NRGN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NRGN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NRGN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NRGN can be calculated.