Human NEFM ELISA Kit

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  • Alternative name

    Human NF-M ELISA Kit;Human 160 kDa neurofilament protein ELISA Kit;Human neurofilament 3 ELISA Kit;Human neurofilament triplet M protein ELISA Kit;Human NEF3 ELISA Kit;Human NFM ELISA Kit;Human neurofilament medium ELISA Kit;Human neurofilament medium polypeptide ELISA Kit;Human neurofilament, medium polypeptide 150kDa ELISA Kit;Human neurofilament-3 (150 kD medium) ELISA Kit;

  • Catalog
    E026159
  • species
    Human
  • GeneNEFM
  • Standard CurveHuman NEFM ELISA Kit
  • Other Species Mouse Nefm ELISA KitBovine NEFM ELISA KitChicken NEFM ELISA KitPorcine NEFM ELISA KitRabbit NEFM ELISA KitRat Nefm ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NEFM. No significant cross-reactivity or interference between NEFM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NEFM and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman NEFM ELISA Kit allows for the in vitro quantitative determination of NEFM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeuroscience
  • Product Description
    specifical
    Intended Uses||This NEFM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NEFM. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: NEFM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NEFM antibody and an NEFM-HRP conjugate. The assay sample and buffer are incubated together with NEFM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEFM concentration since NEFM from samples and NEFM-HRP conjugate compete for the anti-NEFM antibody binding site. Since the number of sites is limited, as more sites are occupied by NEFM from the sample, fewer sites are left to bind NEFM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFM concentration in each sample is interpolated from this standard curve.
  • Human Neurofilament medium polypeptide Protein information
  • Uniprot ID NFM_HUMAN
  • Uniprot AC P07197; B4DGN2; E9PBF7; Q4QRK6;
  • UniGene Hs.458657; Hs.730072;
  • GeneID 4741
  • KEGG hsa:4741;
  • Human Neurofilament medium polypeptide Protein SEQUENCE
  • SEQUENCE 916 AA; 102472 MW; 187B16CA6C6BF092 CRC64;

    MSYTLDSLGN PSAYRRVTET RSSFSRVSGS PSSGFRSQSW SRGSPSTVSS

    SYKRSMLAPR LAYSSAMLSS AESSLDFSQS SSLLNGGSGP GGDYKLSRSN

    EKEQLQGLND RFAGYIEKVH YLEQQNKEIE AEIQALRQKQ ASHAQLGDAY

    DQEIRELRAT LEMVNHEKAQ VQLDSDHLEE DIHRLKERFE EEARLRDDTE

    AAIRALRKDI EEASLVKVEL DKKVQSLQDE VAFLRSNHEE EVADLLAQIQ

    ASHITVERKD YLKTDISTAL KEIRSQLESH SDQNMHQAEE WFKCRYAKLT

    EAAEQNKEAI RSAKEEIAEY RRQLQSKSIE LESVRGTKES LERQLSDIEE

    RHNHDLSSYQ DTIQQLENEL RGTKWEMARH LREYQDLLNV KMALDIEIAA

    YRKLLEGEET RFSTFAGSIT GPLYTHRPPI TISSKIQKPK VEAPKLKVQH

    KFVEEIIEET KVEDEKSEME EALTAITEEL AVSMKEEKKE AAEEKEEEPE

    AEEEEVAAKK SPVKATAPEV KEEEGEKEEE EGQEEEEEED EGAKSDQAEE

    GGSEKEGSSE KEEGEQEEGE TEAEAEGEEA EAKEEKKVEE KSEEVATKEE

    LVADAKVEKP EKAKSPVPKS PVEEKGKSPV PKSPVEEKGK SPVPKSPVEE

    KGKSPVPKSP VEEKGKSPVS KSPVEEKAKS PVPKSPVEEA KSKAEVGKGE

    QKEEEEKEVK EAPKEEKVEK KEEKPKDVPE KKKAESPVKE EAVAEVVTIT

    KSVKVHLEKE TKEEGKPLQQ EKEKEKAGGE GGSEEEGSDK GAKGSRKEDI

    AVNGEVEGKE EVEQETKEKG SGREEEKGVV TNGLDLSPAD EKKGGDKSEE

    KVVVTKTVEK ITSEGGDGAT KYITKSVTVT QKVEEHEETF EEKLVSTKKV

    EKVTSHAIVK EVTQSD

  • UCSC uc003xed.5; human. [P07197-1];


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