Human NEFM ELISA Kit

Alternative name
Human NF-M ELISA Kit;Human 160 kDa neurofilament protein ELISA Kit;Human neurofilament 3 ELISA Kit;Human neurofilament triplet M protein ELISA Kit;Human NEF3 ELISA Kit;Human NFM ELISA Kit;Human neurofilament medium ELISA Kit;Human neurofilament medium polypeptide ELISA Kit;Human neurofilament, medium polypeptide 150kDa ELISA Kit;Human neurofilament-3 (150 kD medium) ELISA Kit;
Catalog
E026159
species
Human
GeneNEFM
SpecificityThis assay has high sensitivity and excellent specificity for detection of NEFM. No significant cross-reactivity or interference between NEFM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NEFM and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman NEFM ELISA Kit allows for the in vitro quantitative determination of NEFM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalIntended Uses||This NEFM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NEFM. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: NEFM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NEFM antibody and an NEFM-HRP conjugate. The assay sample and buffer are incubated together with NEFM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEFM concentration since NEFM from samples and NEFM-HRP conjugate compete for the anti-NEFM antibody binding site. Since the number of sites is limited, as more sites are occupied by NEFM from the sample, fewer sites are left to bind NEFM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFM concentration in each sample is interpolated from this standard curve.
Human Neurofilament medium polypeptide Protein information
Uniprot ID NFM_HUMAN
Uniprot AC P07197; B4DGN2; E9PBF7; Q4QRK6;
UniGene Hs.458657; Hs.730072;
GeneID 4741
KEGG hsa:4741;
Human Neurofilament medium polypeptide Protein SEQUENCE
SEQUENCE 916 AA; 102472 MW; 187B16CA6C6BF092 CRC64;
MSYTLDSLGN PSAYRRVTET RSSFSRVSGS PSSGFRSQSW SRGSPSTVSS
SYKRSMLAPR LAYSSAMLSS AESSLDFSQS SSLLNGGSGP GGDYKLSRSN
EKEQLQGLND RFAGYIEKVH YLEQQNKEIE AEIQALRQKQ ASHAQLGDAY
DQEIRELRAT LEMVNHEKAQ VQLDSDHLEE DIHRLKERFE EEARLRDDTE
AAIRALRKDI EEASLVKVEL DKKVQSLQDE VAFLRSNHEE EVADLLAQIQ
ASHITVERKD YLKTDISTAL KEIRSQLESH SDQNMHQAEE WFKCRYAKLT
EAAEQNKEAI RSAKEEIAEY RRQLQSKSIE LESVRGTKES LERQLSDIEE
RHNHDLSSYQ DTIQQLENEL RGTKWEMARH LREYQDLLNV KMALDIEIAA
YRKLLEGEET RFSTFAGSIT GPLYTHRPPI TISSKIQKPK VEAPKLKVQH
KFVEEIIEET KVEDEKSEME EALTAITEEL AVSMKEEKKE AAEEKEEEPE
AEEEEVAAKK SPVKATAPEV KEEEGEKEEE EGQEEEEEED EGAKSDQAEE
GGSEKEGSSE KEEGEQEEGE TEAEAEGEEA EAKEEKKVEE KSEEVATKEE
LVADAKVEKP EKAKSPVPKS PVEEKGKSPV PKSPVEEKGK SPVPKSPVEE
KGKSPVPKSP VEEKGKSPVS KSPVEEKAKS PVPKSPVEEA KSKAEVGKGE
QKEEEEKEVK EAPKEEKVEK KEEKPKDVPE KKKAESPVKE EAVAEVVTIT
KSVKVHLEKE TKEEGKPLQQ EKEKEKAGGE GGSEEEGSDK GAKGSRKEDI
AVNGEVEGKE EVEQETKEKG SGREEEKGVV TNGLDLSPAD EKKGGDKSEE
KVVVTKTVEK ITSEGGDGAT KYITKSVTVT QKVEEHEETF EEKLVSTKKV
EKVTSHAIVK EVTQSD
UCSC uc003xed.5; human. [P07197-1];

Category

Human NEFM ELISA Kit