Human GNAS ELISA Kit

Alternative name
Human NESP55 ELISA Kit;Human AHO ELISA Kit;Human C20orf45 ELISA Kit;Human GNAS1 ELISA Kit;Human GPSA ELISA Kit;Human GSA ELISA Kit;Human GSP ELISA Kit;Human NESP ELISA Kit;Human POH ELISA Kit;Human SCG6 ELISA Kit;Human SgVI ELISA Kit;Human GNAS complex locus ELISA Kit;Human protein ALEX ELISA Kit;Human protein GNAS ELISA Kit;Human protein SCG6 (secretogranin VI) ELISA Kit;Human G protein subunit alpha S ELISA Kit;Human adenylate cyclase-stimulating G alpha protein ELISA Kit;Human alternative gene product encoded by XL-exon ELISA Kit;Human extra large alphas protein ELISA Kit;Human guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 ELISA Kit;Human guanine nucleotide regulatory protein ELISA Kit;Human guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas ELISA Kit;Human neuroendocrine secretory protein ELISA Kit;Human secretogranin VI ELISA Kit;
Catalog
E026137
species
Human
GeneGNAS
SpecificityThis assay has high sensitivity and excellent specificity for detection of GNAS. No significant cross-reactivity or interference between GNAS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GNAS and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GNAS ELISA Kit allows for the in vitro quantitative determination of GNAS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This GNAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GNAS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GNAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GNAS antibody and an GNAS-HRP conjugate. The assay sample and buffer are incubated together with GNAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GNAS concentration since GNAS from samples and GNAS-HRP conjugate compete for the anti-GNAS antibody binding site. Since the number of sites is limited, as more sites are occupied by GNAS from the sample, fewer sites are left to bind GNAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GNAS concentration in each sample is interpolated from this standard curve.
Human GPIPIRRH peptide Protein information
Uniprot ID GNAS3_HUMAN
Uniprot AC O95467; B2RB88; E1P5G2; O95417;
UniGene Hs.125898;
GeneID 2778
KEGG hsa:2778;
Human GPIPIRRH peptide Protein SEQUENCE
SEQUENCE 245 AA; 28029 MW; 4F02B8B1115089E2 CRC64;
MDRRSRAQQW RRARHNYNDL CPPIGRRAAT ALLWLSCSIA LLRALATSNA
RAQQRAAAQQ RRSFLNAHHR SGAQVFPESP ESESDHEHEE ADLELSLPEC
LEYEEEFDYE TESETESEIE SETDFETEPE TAPTTEPETE PEDDRGPVVP
KHSTFGQSLT QRLHALKLRS PDASPSRAPP STQEPQSPRE GEELKPEDKD
PRDPEESKEP KEEKQRRRCK PKKPTRRDAS PESPSKKGPI PIRRH
UCSC uc002xzt.5; human. [O95467-1];

Category

Human GNAS ELISA Kit