Human GNAS ELISA Kit

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  • Alternative name

    Human NESP55 ELISA Kit;Human AHO ELISA Kit;Human C20orf45 ELISA Kit;Human GNAS1 ELISA Kit;Human GPSA ELISA Kit;Human GSA ELISA Kit;Human GSP ELISA Kit;Human NESP ELISA Kit;Human POH ELISA Kit;Human SCG6 ELISA Kit;Human SgVI ELISA Kit;Human GNAS complex locus ELISA Kit;Human protein ALEX ELISA Kit;Human protein GNAS ELISA Kit;Human protein SCG6 (secretogranin VI) ELISA Kit;Human G protein subunit alpha S ELISA Kit;Human adenylate cyclase-stimulating G alpha protein ELISA Kit;Human alternative gene product encoded by XL-exon ELISA Kit;Human extra large alphas protein ELISA Kit;Human guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 ELISA Kit;Human guanine nucleotide regulatory protein ELISA Kit;Human guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas ELISA Kit;Human neuroendocrine secretory protein ELISA Kit;Human secretogranin VI ELISA Kit;

  • Catalog
    E026137
  • species
    Human
  • GeneGNAS
  • Standard CurveHuman GNAS ELISA Kit
  • Other Species Mouse Gnas ELISA KitRat Gnas ELISA KitBovine GNAS ELISA KitCanine GNAS ELISA KitHamster GNAS ELISA KitPorcine GNAS ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GNAS. No significant cross-reactivity or interference between GNAS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GNAS and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GNAS ELISA Kit allows for the in vitro quantitative determination of GNAS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This GNAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GNAS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GNAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GNAS antibody and an GNAS-HRP conjugate. The assay sample and buffer are incubated together with GNAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GNAS concentration since GNAS from samples and GNAS-HRP conjugate compete for the anti-GNAS antibody binding site. Since the number of sites is limited, as more sites are occupied by GNAS from the sample, fewer sites are left to bind GNAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GNAS concentration in each sample is interpolated from this standard curve.
  • Human GPIPIRRH peptide Protein information
  • Uniprot ID GNAS3_HUMAN
  • Uniprot AC O95467; B2RB88; E1P5G2; O95417;
  • UniGene Hs.125898;
  • GeneID 2778
  • KEGG hsa:2778;
  • Human GPIPIRRH peptide Protein SEQUENCE
  • SEQUENCE 245 AA; 28029 MW; 4F02B8B1115089E2 CRC64;

    MDRRSRAQQW RRARHNYNDL CPPIGRRAAT ALLWLSCSIA LLRALATSNA

    RAQQRAAAQQ RRSFLNAHHR SGAQVFPESP ESESDHEHEE ADLELSLPEC

    LEYEEEFDYE TESETESEIE SETDFETEPE TAPTTEPETE PEDDRGPVVP

    KHSTFGQSLT QRLHALKLRS PDASPSRAPP STQEPQSPRE GEELKPEDKD

    PRDPEESKEP KEEKQRRRCK PKKPTRRDAS PESPSKKGPI PIRRH

  • UCSC uc002xzt.5; human. [O95467-1];


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