SensitivityThe minimum detectable dose of human NEDD8 is typically less than 9.77 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman NEDD8 ELISA Kit allows for the in vitro quantitative determination of NEDD8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NEDD8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEDD8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEDD8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEDD8 bound in the initial step. The color development is stopped and the intensity of the color is measured.