Human NES ELISA Kit

Alternative name
Human Nbla00170 ELISA Kit;Human nestin ELISA Kit;
Catalog
E026003
species
Human
GeneNES
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nestin (NES). No significant cross-reactivity or interference between Nestin (NES) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman NES ELISA Kit allows for the in vitro quantitative determination of NES , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nestin (NES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nestin (NES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nestin (NES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nestin (NES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Nestin Protein information
Uniprot ID NEST_HUMAN
Uniprot AC P48681; O00552; Q3LIF5; Q5SYZ6;
UniGene Hs.527971;
GeneID 10763
KEGG hsa:10763;
Human Nestin Protein SEQUENCE
SEQUENCE 1621 AA; 177439 MW; 0E4F967C11F44C13 CRC64;
MEGCMGEESF QMWELNRRLE AYLARVKALE EQNELLSAEL GGLRAQSADT
SWRAHADDEL AALRALVDQR WREKHAAEVA RDNLAEELEG VAGRCQQLRL
ARERTTEEVA RNRRAVEAEK CARAWLSSQV AELERELEAL RVAHEEERVG
LNAQAACAPR CPAPPRGPPA PAPEVEELAR RLGEAWRGAV RGYQERVAHM
ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA ALEQRLEGRW
QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA
TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV
LSPTSLPSPL PATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSPA
VDAEIRAQDA PLSLLQTQGG RKQAPEPLRA EARVAIPASV LPGPEEPGGQ
RQEASTGQSP EDHASLAPPL SPDHSSLEAK DGESGGSRVF SICRGEGEGQ
IWGLVEKETA IEGKVVSSLQ QEIWEEEDLN RKEIQDSQVP LEKETLKSLG
EEIQESLKTL ENQSHETLER ENQECPRSLE EDLETLKSLE KENKELLKDV
EVVRPLEKEA VGQLKPTGKE DTQTLQSLQK ENQELMKSLE GNLETFLFPG
TENQELVSSL QENLESLTAL EKENQEPLRS PEVGDEEALR PLTKENQEPL
RSLEDENKEA FRSLEKENQE PLKTLEEEDQ SIVRPLETEN HKSLRSLEEQ
DQETLRTLEK ETQQRRRSLG EQDQMTLRPP EKVDLEPLKS LDQEIARPLE
NENQEFLKSL KEESVEAVKS LETEILESLK SAGQENLETL KSPETQAPLW
TPEEINQGAM NPLEKEIQEP LESVEVNQET FRLLEEENQE SLRSLGAWNL
ENLRSPEEVD KESQRNLEEE ENLGKGEYQE SLRSLEEEGQ ELPQSADVQR
WEDTVEKDQE LAQESPPGMA GVENEDEAEL NLREQDGFTG KEEVVEQGEL
NATEEVWIPG EGHPESPEPK EQRGLVEGAS VKGGAEGLQD PEGQSQQVGA
PGLQAPQGLP EAIEPLVEDD VAPGGDQASP EVMLGSEPAM GESAAGAEPG
PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP RKDLEEAGGL
GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP
SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ
GRAEALGKVE SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP
LGFYLRSPTS PRWDPTGEQR PPPQGETGKE GWDPAVLASE GLEAPPSEKE
EGEEGEEECG RDSDLSEEFE DLGTEAPFLP GVPGEVAEPL GQVPQLLLDP
AAWDRDGESD GFADEEESGE EGEEDQEEGR EPGAGRWGPG SSVGSLQALS
SSQRGEFLES DSVSVSVPWD DSLRGAVAGA PKTALETESQ DSAEPSGSEE
ESDPVSLERE DKVPGPLEIP SGMEDAGPGA DIIGVNGQGP NLEGKSQHVN
GGVMNGLEQS EEVGQGMPLV SEGDRGSPFQ EEEGSALKTS WAGAPVHLGQ
GQFLKFTQRE GDRESWSSGE D
UCSC uc001fpq.4; human.;

Category

Human NES ELISA Kit

Human Nbla00170 ELISA Kit;Human nestin ELISA Kit;