Human NES ELISA Kit

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  • Alternative name

    Human Nbla00170 ELISA Kit;Human nestin ELISA Kit;

  • Catalog
    E026003
  • species
    Human
  • GeneNES
  • Standard CurveHuman NES ELISA Kit
  • Other Species Human Nes-1 ELISA KitMouse Nes ELISA KitRat Nes ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nestin (NES). No significant cross-reactivity or interference between Nestin (NES) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman NES ELISA Kit allows for the in vitro quantitative determination of NES , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nestin (NES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nestin (NES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nestin (NES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nestin (NES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nestin Protein information
  • Uniprot ID NEST_HUMAN
  • Uniprot AC P48681; O00552; Q3LIF5; Q5SYZ6;
  • UniGene Hs.527971;
  • GeneID 10763
  • KEGG hsa:10763;
  • Human Nestin Protein SEQUENCE
  • SEQUENCE 1621 AA; 177439 MW; 0E4F967C11F44C13 CRC64;

    MEGCMGEESF QMWELNRRLE AYLARVKALE EQNELLSAEL GGLRAQSADT

    SWRAHADDEL AALRALVDQR WREKHAAEVA RDNLAEELEG VAGRCQQLRL

    ARERTTEEVA RNRRAVEAEK CARAWLSSQV AELERELEAL RVAHEEERVG

    LNAQAACAPR CPAPPRGPPA PAPEVEELAR RLGEAWRGAV RGYQERVAHM

    ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA ALEQRLEGRW

    QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA

    TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV

    LSPTSLPSPL PATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSPA

    VDAEIRAQDA PLSLLQTQGG RKQAPEPLRA EARVAIPASV LPGPEEPGGQ

    RQEASTGQSP EDHASLAPPL SPDHSSLEAK DGESGGSRVF SICRGEGEGQ

    IWGLVEKETA IEGKVVSSLQ QEIWEEEDLN RKEIQDSQVP LEKETLKSLG

    EEIQESLKTL ENQSHETLER ENQECPRSLE EDLETLKSLE KENKELLKDV

    EVVRPLEKEA VGQLKPTGKE DTQTLQSLQK ENQELMKSLE GNLETFLFPG

    TENQELVSSL QENLESLTAL EKENQEPLRS PEVGDEEALR PLTKENQEPL

    RSLEDENKEA FRSLEKENQE PLKTLEEEDQ SIVRPLETEN HKSLRSLEEQ

    DQETLRTLEK ETQQRRRSLG EQDQMTLRPP EKVDLEPLKS LDQEIARPLE

    NENQEFLKSL KEESVEAVKS LETEILESLK SAGQENLETL KSPETQAPLW

    TPEEINQGAM NPLEKEIQEP LESVEVNQET FRLLEEENQE SLRSLGAWNL

    ENLRSPEEVD KESQRNLEEE ENLGKGEYQE SLRSLEEEGQ ELPQSADVQR

    WEDTVEKDQE LAQESPPGMA GVENEDEAEL NLREQDGFTG KEEVVEQGEL

    NATEEVWIPG EGHPESPEPK EQRGLVEGAS VKGGAEGLQD PEGQSQQVGA

    PGLQAPQGLP EAIEPLVEDD VAPGGDQASP EVMLGSEPAM GESAAGAEPG

    PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP RKDLEEAGGL

    GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP

    SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ

    GRAEALGKVE SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP

    LGFYLRSPTS PRWDPTGEQR PPPQGETGKE GWDPAVLASE GLEAPPSEKE

    EGEEGEEECG RDSDLSEEFE DLGTEAPFLP GVPGEVAEPL GQVPQLLLDP

    AAWDRDGESD GFADEEESGE EGEEDQEEGR EPGAGRWGPG SSVGSLQALS

    SSQRGEFLES DSVSVSVPWD DSLRGAVAGA PKTALETESQ DSAEPSGSEE

    ESDPVSLERE DKVPGPLEIP SGMEDAGPGA DIIGVNGQGP NLEGKSQHVN

    GGVMNGLEQS EEVGQGMPLV SEGDRGSPFQ EEEGSALKTS WAGAPVHLGQ

    GQFLKFTQRE GDRESWSSGE D

  • UCSC uc001fpq.4; human.;


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