Human NUCB2 ELISA Kit

Alternative name
Human DNA-binding protein NEFA ELISA Kit;Human gastric cancer antigen Zg4 ELISA Kit;Human prepronesfatin ELISA Kit;Human NEFA ELISA Kit;Human HEL-S-109 ELISA Kit;Human nucleobindin 2 ELISA Kit;Human nucleobindin-2 ELISA Kit;Human epididymis secretory protein Li 109 ELISA Kit;Human nesfatin 1 ELISA Kit;Human nucleobinding 2 ELISA Kit;Human prepronefastin ELISA Kit;
Catalog
E025987
species
Human
GeneNUCB2
SpecificityNatural and recombinant human Nesfatin-1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman NUCB2 ELISA Kit allows for the in vitro quantitative determination of NUCB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Nesfatin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nesfatin-1 has been precoated onto 96-well plates. Standards(E.coli, V25-L106) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nesfatin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Nesfatin-1 amount of sample captured in plate. Background: Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
Human Nesfatin-1 Protein information
Uniprot ID NUCB2_HUMAN
Uniprot AC P80303; A8K642; D3DQX5; Q8NFT5;
UniGene Hs.654599;
GeneID 4925
KEGG hsa:4925;
Human Nesfatin-1 Protein SEQUENCE
SEQUENCE 420 AA; 50196 MW; 5BA7459AB725A1A3 CRC64;
MRWRTILLQY CFLLITCLLT ALEAVPIDID KTKVQNIHPV ESAKIEPPDT
GLYYDEYLKQ VIDVLETDKH FREKLQKADI EEIKSGRLSK ELDLVSHHVR
TKLDELKRQE VGRLRMLIKA KLDSLQDIGM DHQALLKQFD HLNHLNPDKF
ESTDLDMLIK AATSDLEHYD KTRHEEFKKY EMMKEHERRE YLKTLNEEKR
KEEESKFEEM KKKHENHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF
KLHDVNSDGF LDEQELEALF TKELEKVYDP KNEEDDMVEM EEERLRMREH
VMSEVDTNKD RLVTLEEFLK ATEKKEFLEP DSWETLDQQQ FFTEEELKEY
ENIIALQENE LKKKADELQK QKEELQRQHD QLEAQKLEYH QVIQQMEQKK
LQQGIPPSGP AGELKFEPHI

Category

Human NUCB2 ELISA Kit