Human NGF ELISA Kit

Alternative name
Human Beta-NGF ELISA Kit;Human NGFB ELISA Kit;Human HSAN5 ELISA Kit;Human nerve growth factor ELISA Kit;Human beta-nerve growth factor ELISA Kit;Human nerve growth factor (beta polypeptide) ELISA Kit;Human nerve growth factor, beta subunit ELISA Kit;
Catalog
E025973
species
Human
GeneNGF
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF). No significant cross-reactivity or interference between Nerve Growth Factor (NGF) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 5.7pg/mL.
Intended UseHuman NGF ELISA Kit allows for the in vitro quantitative determination of NGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor (NGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nerve Growth Factor (NGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nerve Growth Factor (NGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nerve Growth Factor (NGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Beta-nerve growth factor Protein information
Uniprot ID NGF_HUMAN
Uniprot AC P01138; A1A4E5; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8;
UniGene Hs.2561;
GeneID 4803
KEGG hsa:4803;
Human Beta-nerve growth factor Protein SEQUENCE
SEQUENCE 241 AA; 26959 MW; 619DFC65EB3BD671 CRC64;
MSMLFYTLIT AFLIGIQAEP HSESNVPAGH TIPQAHWTKL QHSLDTALRR
ARSAPAAAIA ARVAGQTRNI TVDPRLFKKR RLRSPRVLFS TQPPREAADT
QDLDFEVGGA APFNRTHRSK RSSSHPIFHR GEFSVCDSVS VWVGDKTTAT
DIKGKEVMVL GEVNINNSVF KQYFFETKCR DPNPVDSGCR GIDSKHWNSY
CTTTHTFVKA LTMDGKQAAW RFIRIDTACV CVLSRKAVRR A
UCSC uc001efu.2; human.;

Category

Human NGF ELISA Kit

Human Beta-NGF ELISA Kit;Human NGFB ELISA Kit;Human HSAN5 ELISA Kit;Human nerve growth factor ELISA Kit;Human beta-nerve growth factor ELISA Kit;Human nerve growth factor (beta polypeptide) ELISA Kit;Human nerve growth factor, beta subunit ELISA Kit;