Human NEIL1 ELISA Kit

Alternative name
Human DNA glycosylase/AP lyase Neil1 ELISA Kit;Human DNA-(apurinic or apyrimidinic site) lyase Neil1 ELISA Kit;Human endonuclease VIII-like 1 ELISA Kit;Human FPG1 ELISA Kit;Human nei homolog 1 ELISA Kit;Human NEH1 ELISA Kit;Human nei-like protein 1 ELISA Kit;Human NEI1 ELISA Kit;Human hFPG1 ELISA Kit;Human nei like DNA glycosylase 1 ELISA Kit;Human endonuclease 8-like 1 ELISA Kit;Human DNA endonuclease eight-like glycosylase 1 ELISA Kit;Human endonuclease VIII ELISA Kit;Human nei endonuclease VIII-like 1 ELISA Kit;
Catalog
E025922
species
Human
GeneNEIL1
Standard Curve
Other Species Mouse Neil1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nei Endonuclease VIII Like Protein 1 (NEIL1). No significant cross-reactivity or interference between Nei Endonuclease VIII Like Protein 1 (NEIL1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman NEIL1 ELISA Kit allows for the in vitro quantitative determination of NEIL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nei Endonuclease VIII Like Protein 1 (NEIL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nei Endonuclease VIII Like Protein 1 (NEIL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Endonuclease 8-like 1 Protein information
Uniprot ID NEIL1_HUMAN
Uniprot AC Q96FI4; D3DW75; Q6ZRA7; Q86XW7; Q9H6C3;
UniGene Hs.512732;
GeneID 79661
KEGG hsa:79661;
Human Endonuclease 8-like 1 Protein SEQUENCE
SEQUENCE 390 AA; 43684 MW; B2B058486C4EF835 CRC64;
MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS
ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR
FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR
NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA
LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE
EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS
KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE
APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS
UCSC uc002bad.5; human.;

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