Human NAPSA ELISA Kit

Alternative name
Human aspartyl protease 4 ELISA Kit;Human ASP4 ELISA Kit;Human asp 4 ELISA Kit;Human napsin-1 ELISA Kit;Human TA01/TA02 ELISA Kit;Human NAP1 ELISA Kit;Human NAPA ELISA Kit;Human KAP ELISA Kit;Human Kdap ELISA Kit;Human SNAPA ELISA Kit;Human napsin A aspartic peptidase ELISA Kit;Human napsin-A ELISA Kit;Human kidney-derived aspartic protease-like protein ELISA Kit;Human pronapsin A ELISA Kit;
Catalog
E025846
species
Human
GeneNAPSA
SpecificityThis assay has high sensitivity and excellent specificity for detection of human NAPSA. No significant cross-reactivity or interference between human NAPSA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NAPSA is typically less than 1.18 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman NAPSA ELISA Kit allows for the in vitro quantitative determination of NAPSA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAPSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAPSA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAPSA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAPSA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Napsin-A Protein information
Uniprot ID NAPSA_HUMAN
Uniprot AC O96009; Q8WWD9;
UniGene Hs.512843;
GeneID 9476
KEGG hsa:9476;
Human Napsin-A Protein SEQUENCE
SEQUENCE 420 AA; 45387 MW; 018B86AE5BD0C865 CRC64;
MSPPPLLQPL LLLLPLLNVE PSGATLIRIP LHRVQPGRRI LNLLRGWREP
AELPKLGAPS PGDKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS
NLWVPSRRCH FFSVPCWLHH RFDPKASSSF QANGTKFAIQ YGTGRVDGIL
SEDKLTIGGI KGASVIFGEA LWEPSLVFAF AHFDGILGLG FPILSVEGVR
PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP AHYIPPLTFV
PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH
AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG
VRLCLSGFQA LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR
ARTRGADLGW GETAQAQFPG
UCSC uc002prx.4; human.;

Category

Human NAPSA ELISA Kit