Intended UseHuman NADPH ELISA Kit allows for the in vitro quantitative determination of NADPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NADPH concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with NADPH. During the reaction, NADPH in the sample or standard competes with a fixed amount of NADPH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NADPH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NADPH in the samples is then determined by comparing the OD of the samples to the standard curve.