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Human NDUFS1 ELISA Kit

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  • Alternative name

    Human Complex I-75kD ELISA Kit;Human CI-75Kd ELISA Kit;Human CI-75k ELISA Kit;Human PRO1304 ELISA Kit;Human NADH:ubiquinone oxidoreductase core subunit S1 ELISA Kit;Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial ELISA Kit;Human NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) ELISA Kit;Human complex I 75kDa subunit ELISA Kit;Human complex I, mitochondrial respiratory chain, 75-kD subunit ELISA Kit;Human mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit ELISA Kit;
  • Catalog
    E025795
  • species
    Human
  • GeneNDUFS1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1). No significant cross-reactivity or interference between NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.115ng/mL.
  • Intended UseHuman NDUFS1 ELISA Kit allows for the in vitro quantitative determination of NDUFS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Protein information
  • Uniprot ID NDUS1_HUMAN
  • Uniprot AC Q8TCC9;
  • UniGene Hs.471207; Hs.598436;
  • GeneID 4719
  • KEGG hsa:4719;
  • Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Protein SEQUENCE

  • SEQUENCE 727 AA; 79468 MW; 9C35F4B8294771FB CRC64;

    MLRIPVRKAL VGLSKSPKGC VRTTATAASN LIEVFVDGQS VMVEPGTTVL

    QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW

    NILTNSEKSK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGNDR

    SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR

    GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES

    IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ

    RLTEPMVRNE KGLLTYTSWE DALSRVAGML QSFQGKDVAA IAGGLVDAEA

    LVALKDLLNR VDSDTLCTEE VFPTAGAGTD LRSNYLLNTT IAGVEEADVV

    LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY TYDHLGDSPK

    ILQDIASGSH PFSQVLKEAK KPMVVLGSSA LQRNDGAAIL AAVSSIAQKI

    RMTSGVTGDW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPKVLFLLGA

    DGGCITRQDL PKDCFIIYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE

    GRAQQTKVAV TPPGLAREDW KIIRALSEIA GMTLPYDTLD QVRNRLEEVS

    PNLVRYDDIE GANYFQQANE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI

    SRASQTMAKC VKAVTEGAQA VEEPSIC

  • UCSC uc002vbe.4; human. [P28331-1];



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