Human NDUFS1 ELISA Kit

Alternative name
Human Complex I-75kD ELISA Kit;Human CI-75Kd ELISA Kit;Human CI-75k ELISA Kit;Human PRO1304 ELISA Kit;Human NADH:ubiquinone oxidoreductase core subunit S1 ELISA Kit;Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial ELISA Kit;Human NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) ELISA Kit;Human complex I 75kDa subunit ELISA Kit;Human complex I, mitochondrial respiratory chain, 75-kD subunit ELISA Kit;Human mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit ELISA Kit;
Catalog
E025795
species
Human
GeneNDUFS1
Standard Curve
Other Species Mouse Ndufs1 ELISA Kit Bovine NDUFS1 ELISA Kit Monkey NDUFS1 ELISA Kit Rat Ndufs1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1). No significant cross-reactivity or interference between NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.115ng/mL.
Intended UseHuman NDUFS1 ELISA Kit allows for the in vitro quantitative determination of NDUFS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Protein information
Uniprot ID NDUS1_HUMAN
Uniprot AC Q8TCC9;
UniGene Hs.471207; Hs.598436;
GeneID 4719
KEGG hsa:4719;
Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Protein SEQUENCE
SEQUENCE 727 AA; 79468 MW; 9C35F4B8294771FB CRC64;
MLRIPVRKAL VGLSKSPKGC VRTTATAASN LIEVFVDGQS VMVEPGTTVL
QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW
NILTNSEKSK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGNDR
SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR
GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES
IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ
RLTEPMVRNE KGLLTYTSWE DALSRVAGML QSFQGKDVAA IAGGLVDAEA
LVALKDLLNR VDSDTLCTEE VFPTAGAGTD LRSNYLLNTT IAGVEEADVV
LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY TYDHLGDSPK
ILQDIASGSH PFSQVLKEAK KPMVVLGSSA LQRNDGAAIL AAVSSIAQKI
RMTSGVTGDW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPKVLFLLGA
DGGCITRQDL PKDCFIIYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE
GRAQQTKVAV TPPGLAREDW KIIRALSEIA GMTLPYDTLD QVRNRLEEVS
PNLVRYDDIE GANYFQQANE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI
SRASQTMAKC VKAVTEGAQA VEEPSIC
UCSC uc002vbe.4; human. [P28331-1];

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Human NDUFS1 ELISA Kit