Human ND5 ELISA Kit

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  • Alternative name

    NADH-ubiquinone oxidoreductase chain 5 ELISA KIT; NADH dehydrogenase subunit 5MT-ND5 ELISA KIT; MTND5 ELISA KIT; NADH5 ELISA KIT; ND5 ELISA KIT

  • Catalog
  • species
  • GeneND5
  • Standard CurveHuman ND5 ELISA Kit
  • Other Species Human MT ND5 ELISA KitHuman MT-ND5 ELISA KitMouse MT-ND5 ELISA KitBovine MT-ND5 ELISA KitCanine MT-ND5 ELISA KitChicken MT-ND5 ELISA KitHorse MT-ND5 ELISA KitMonkey MT-ND5 ELISA KitPorcine MT-ND5 ELISA KitRabbit MT-ND5 ELISA KitSheep MT-ND5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NADH Dehydrogenase 5 (ND5). No significant cross-reactivity or interference between NADH Dehydrogenase 5 (ND5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 29.6pg/mL.
  • Intended UseHuman ND5 ELISA Kit allows for the in vitro quantitative determination of ND5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase 5 (ND5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase 5 (ND5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase 5 (ND5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NADH Dehydrogenase 5 (ND5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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