Human ND5 ELISA Kit

Contact Us
  • Alternative name

    NADH-ubiquinone oxidoreductase chain 5 ELISA KIT; NADH dehydrogenase subunit 5MT-ND5 ELISA KIT; MTND5 ELISA KIT; NADH5 ELISA KIT; ND5 ELISA KIT

  • Catalog
    E025791
  • species
    Human
  • GeneND5
  • Standard CurveHuman ND5 ELISA Kit
  • Other Species Human MT ND5 ELISA KitHuman MT-ND5 ELISA KitMouse MT-ND5 ELISA KitBovine MT-ND5 ELISA KitCanine MT-ND5 ELISA KitChicken MT-ND5 ELISA KitHorse MT-ND5 ELISA KitMonkey MT-ND5 ELISA KitPorcine MT-ND5 ELISA KitRabbit MT-ND5 ELISA KitSheep MT-ND5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NADH Dehydrogenase 5 (ND5). No significant cross-reactivity or interference between NADH Dehydrogenase 5 (ND5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 29.6pg/mL.
  • Intended UseHuman ND5 ELISA Kit allows for the in vitro quantitative determination of ND5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase 5 (ND5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase 5 (ND5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase 5 (ND5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NADH Dehydrogenase 5 (ND5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



Inquiry About Human ND5 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!