Human SIRT1O ELISA Kit

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  • Alternative name

    NAD-dependent protein deacetylase sirtuin-1 ELISA KIT; Regulatory protein SIR2 homolog 1 ELISA KIT; SIR2-like protein 1 ELISA KIT; hSIR2SIRT1 ELISA KIT; SIR2L1 ELISA KIT; hSIRT1 ELISA KIT; hSIR2 ELISA KIT; 75SirT1 ELISA KIT

  • Catalog
    E025746
  • species
    Human
  • GeneSIRT1O
  • Standard CurveHuman SIRT1O ELISA Kit
  • Other Species Mouse Sirt1O ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural human SIRT1 . No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman SIRT1O ELISA Kit allows for the in vitro quantitative determination of SIRT1O , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRT1 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SIRT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SIRT1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SIRT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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