Human NADK ELISA Kit

Alternative name
Human poly(P)/ATP NAD kinase ELISA Kit;Human dJ283E3.1 ELISA Kit;Human NAD kinase ELISA Kit;
Catalog
E025739
species
Human
GeneNADK
SpecificityThis assay has high sensitivity and excellent specificity for detection of NADK. No significant cross-reactivity or interference between NADK and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NADK is typically less than 0.062ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman NADK ELISA Kit allows for the in vitro quantitative determination of NADK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NADK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human NAD kinase Protein information
Uniprot ID NADK_HUMAN
Uniprot AC O95544; A6NNN3; A8K296; B7Z434; F5GXR5; Q5QPS4; Q9H2P2; Q9H931;
UniGene Hs.654792; Hs.731532; Hs.733075;
GeneID 65220
KEGG hsa:65220;
Human NAD kinase Protein SEQUENCE
SEQUENCE 446 AA; 49228 MW; 48CE7AF05EDD7E8B CRC64;
MEMEQEKMTM NKELSPDAAA YCCSACHGDE TWSYNHPIRG RAKSRSLSAS
PALGSTKEFR RTRSLHGPCP VTTFGPKACV LQNPQTIMHI QDPASQRLTW
NKSPKSVLVI KKMRDASLLQ PFKELCTHLM EENMIVYVEK KVLEDPAIAS
DESFGAVKKK FCTFREDYDD ISNQIDFIIC LGGDGTLLYA SSLFQGSVPP
VMAFHLGSLG FLTPFSFENF QSQVTQVIEG NAAVVLRSRL KVRVVKELRG
KKTAVHNGLG ENGSQAAGLD MDVGKQAMQY QVLNEVVIDR GPSSYLSNVD
VYLDGHLITT VQGDGVIVST PTGSTAYAAA AGASMIHPNV PAIMITPICP
HSLSFRPIVV PAGVELKIML SPEARNTAWV SFDGRKRQEI RHGDSISITT
SCYPLPSICV RDPVSDWFES LAQCLHWNVR KKQAHFEEEE EEEEEG
UCSC uc001aic.3; human. [O95544-1];

Category

Human NADK ELISA Kit

Human poly(P)/ATP NAD kinase ELISA Kit;Human dJ283E3.1 ELISA Kit;Human NAD kinase ELISA Kit;