Human NT-ProCNP ELISA Kit

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  • Catalog
    E025702
  • species
    Human
  • GeneNT-ProCNP
  • Standard CurveHuman NT-ProCNP ELISA Kit
  • Other Species Mouse NT-ProCNP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP). No significant cross-reactivity or interference between N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 5.52pg/mL.
  • Intended UseHuman NT-ProCNP ELISA Kit allows for the in vitro quantitative determination of NT-ProCNP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) and unlabeled N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) (Standards or samples) with the pre-coated antibody specific to N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) in the sample.



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