Human AG2s ELISA Kit

Contact Us
  • Catalog
  • species
  • GeneAG2s
  • Standard CurveHuman AG2s ELISA Kit
  • Other Species Mouse AG2S ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman AG2s ELISA Kit allows for the in vitro quantitative determination of AG2s , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: G2s ELISA kit applies the competitive enzyme immunoassay technique utilizing a Actin antigen and an anti-G2s-HRP conjugate. The assay sample and buffer are incubated together with anti-G2s-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-G2s concentration since anti-G2s from samples and anti-G2s-HRP conjugate compete for the Actin antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-G2s from the sample, fewer sites are left to bind anti-G2s-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-G2s concentration in each sample is interpolated from this standard curve.

Inquiry About Human AG2s ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!