Human MPG ELISA Kit

Alternative name
Human 3-alkyladenine DNA glycosylase ELISA Kit;Human 3-methyladenine DNA glycosidase ELISA Kit;Human ADPG ELISA Kit;Human N-methylpurine-DNA glycosylase ELISA Kit;Human AAG ELISA Kit;Human anpg ELISA Kit;Human Mid1 ELISA Kit;Human APNG ELISA Kit;Human CRA36.1 ELISA Kit;Human MDG ELISA Kit;Human PIG11 ELISA Kit;Human PIG16 ELISA Kit;Human N-methylpurine DNA glycosylase ELISA Kit;Human DNA-3-methyladenine glycosylase ELISA Kit;Human 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p ELISA Kit;Human CRA36.1 (3-methyl-adenine DNA glycosylase) ELISA Kit;Human N-methylpurine-DNA glycosylase, MPG ELISA Kit;Human proliferation-inducing protein 11 ELISA Kit;Human proliferation-inducing protein 16 ELISA Kit;
Catalog
E025676
species
Human
GeneMPG
SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Methylpurine DNA Glycosylase (MPG). No significant cross-reactivity or interference between N-Methylpurine DNA Glycosylase (MPG) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman MPG ELISA Kit allows for the in vitro quantitative determination of MPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Methylpurine DNA Glycosylase (MPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Methylpurine DNA Glycosylase (MPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Methylpurine DNA Glycosylase (MPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Methylpurine DNA Glycosylase (MPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human DNA-3-methyladenine glycosylase Protein information
Uniprot ID 3MG_HUMAN
Uniprot AC Q9NNX5;
UniGene Hs.459596;
GeneID 4350
KEGG hsa:4350;
Human DNA-3-methyladenine glycosylase Protein SEQUENCE
SEQUENCE 298 AA; 32869 MW; BEA8C4CB250D572B CRC64;
MVTPALQMKK PKQFCRRMGQ KKQRPARAGQ PHSSSDAAQA PAEQPHSSSD
AAQAPCPRER CLGPPTTPGP YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR
AFLGQVLVRR LPNGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF
MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGL ETMRQLRSTL
RKGTASRVLK DRELCSGPSK LCQALAINKS FDQRDLAQDE AVWLERGPLE
PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDTQA
UCSC uc002cfm.4; human. [P29372-1];

Category

Human MPG ELISA Kit