Human NGLY1 ELISA Kit

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  • Alternative name

    Human PNGase ELISA Kit;Human hPNGase ELISA Kit;Human N-glycanase 1 ELISA Kit;Human peptide:N-glycanase ELISA Kit;Human PNG1 ELISA Kit;Human CDDG ELISA Kit;Human CDG1V ELISA Kit;Human peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase ELISA Kit;

  • Catalog
    E025667
  • species
    Human
  • GeneNGLY1
  • Other Species Chicken NGLY1 ELISA KitMonkey NGLY1 ELISA KitMouse Ngly1 ELISA KitRat Ngly1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NGLY1. No significant cross-reactivity or interference between human NGLY1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NGLY1 is typically less than 9.38 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman NGLY1 ELISA Kit allows for the in vitro quantitative determination of NGLY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGLY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGLY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGLY1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGLY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein information
  • Uniprot ID NGLY1_HUMAN
  • Uniprot AC Q96IV0; B4DJE9; Q59FB1; Q6PJD8; Q9BVR8; Q9NR70;
  • UniGene Hs.368960;
  • GeneID 55768
  • KEGG hsa:55768;
  • Human Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein SEQUENCE
  • SEQUENCE 654 AA; 74390 MW; 94BD44316EA66AF6 CRC64;

    MAAAALGSSS GSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPNDEK

    YRSIRIGNTA FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK

    IRDLIAIERS SRLDGSNKSH KVKSSQQPAA STQLPTTPSS NPSGLNQHTR

    NRQGQSSDPP SASTVAADSA ILEVLQSNIQ HVLVYENPAL QEKALACIPV

    QELKRKSQEK LSRARKLDKG INISDEDFLL LELLHWFKEE FFHWVNNVLC

    SKCGGQTRSR DRSLLPSDDE LKWGAKEVED HYCDACQFSN RFPRYNNPEK

    LLETRCGRCG EWANCFTLCC RAVGFEARYV WDYTDHVWTE VYSPSQQRWL

    HCDACEDVCD KPLLYEIGWG KKLSYVIAFS KDEVVDVTWR YSCKHEEVIA

    RRTKVKEALL RDTINGLNKQ RQLFLSENRR KELLQRIIVE LVEFISPKTP

    KPGELGGRIS GSVAWRVARG EMGLQRKETL FIPCENEKIS KQLHLCYNIV

    KDRYVRVSNN NQTISGWENG VWKMESIFRK VETDWHMVYL ARKEGSSFAY

    ISWKFECGSV GLKVDSISIR TSSQTFQTGT VEWKLRSDTA QVELTGDNSL

    HSYADFSGAT EVILEAELSR GDGDVAWQHT QLFRQSLNDH EENCLEIIIK

    FSDL

  • UCSC uc003cdl.4; human. [Q96IV0-1];



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