Human NSF ELISA Kit

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  • Alternative name

    Human N-ethylmaleimide-sensitive fusion protein ELISA Kit;Human NEM-sensitive fusion protein ELISA Kit;Human vesicular-fusion protein NSF ELISA Kit;Human SEC18 ELISA Kit;Human SKD2 ELISA Kit;Human N-ethylmaleimide sensitive factor, vesicle fusing ATPase ELISA Kit;Human vesicle-fusing ATPase ELISA Kit;Human N-ethylmaleimide-sensitive factor-like protein ELISA Kit;

  • Catalog
    E025663
  • species
    Human
  • GeneNSF
  • Standard CurveHuman NSF ELISA Kit
  • Other Species Mouse Nsf ELISA KitHamster NSF ELISA KitRat Nsf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Ethylmaleimide Sensitive Factor (NSF). No significant cross-reactivity or interference between N-Ethylmaleimide Sensitive Factor (NSF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.116ng/mL.
  • Intended UseHuman NSF ELISA Kit allows for the in vitro quantitative determination of NSF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Vesicle-fusing ATPase Protein information
  • Uniprot ID NSF_HUMAN
  • Uniprot AC P46459; A8K2D9; B4DFA2; Q8N6D7; Q9UKZ2;
  • UniGene Hs.431279;
  • GeneID 4905
  • KEGG hsa:4905;
  • Human Vesicle-fusing ATPase Protein SEQUENCE
  • SEQUENCE 744 AA; 82594 MW; BF545A2A0C7EC2F7 CRC64;

    MAGRSMQAAR CPTDELSLTN CAVVNEKDFQ SGQHVIVRTS PNHRYTFTLK

    THPSVVPGSI AFSLPQRKWA GLSIGQEIEV SLYTFDKAKQ CIGTMTIEID

    FLQKKSIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NEKLFGLLVK

    DIEAMDPSIL KGEPATGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK

    TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC

    KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA

    NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV

    VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE

    KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS

    TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA

    SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT

    ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV

    VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD

    VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG

    KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALLREEGASP LDFD

  • UCSC uc002iku.4; human. [P46459-1];



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