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Human NANS ELISA Kit

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  • Alternative name

    Human N-acetylneuraminate synthase ELISA Kit;Human N-acetylneuraminate-9-phosphate synthase ELISA Kit;Human N-acetylneuraminic acid phosphate synthase ELISA Kit;Human N-acetylneuraminic acid synthase ELISA Kit;Human SAS ELISA Kit;Human HEL-S-100 ELISA Kit;Human SEMDCG ELISA Kit;Human SEMDG ELISA Kit;Human sialic acid synthase ELISA Kit;Human epididymis secretory protein Li 100 ELISA Kit;Human sialic acid phosphate synthase ELISA Kit;
  • Catalog
    E025623
  • species
    Human
  • GeneNANS
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Acetylneuraminic Acid Synthase (NANS). No significant cross-reactivity or interference between N-Acetylneuraminic Acid Synthase (NANS) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman NANS ELISA Kit allows for the in vitro quantitative determination of NANS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acetylneuraminic Acid Synthase (NANS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acetylneuraminic Acid Synthase (NANS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acetylneuraminic Acid Synthase (NANS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acetylneuraminic Acid Synthase (NANS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sialic acid synthase Protein information
  • Uniprot ID SIAS_HUMAN
  • Uniprot AC Q9NR45; B2RE98; Q8WUV9; Q9BWS6; Q9NVD4;
  • UniGene Hs.522310; Hs.665191;
  • GeneID 54187
  • KEGG hsa:54187;
  • Human Sialic acid synthase Protein SEQUENCE

  • SEQUENCE 359 AA; 40308 MW; 2E02D47F4F98592F CRC64;

    MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC

    AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR

    YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG

    RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI

    SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH

    SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK

    IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV

    DNHGKKIKS

  • UCSC uc004ayc.4; human.;



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