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Human MARCKS ELISA Kit

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  • Alternative name

    Human MARCKS ELISA Kit;Human protein kinase C substrate, 80 kDa protein, light chain ELISA Kit;Human 80K-L protein ELISA Kit;Human PKCSL ELISA Kit;Human MACS ELISA Kit;Human PRKCSL ELISA Kit;Human 80K-L ELISA Kit;Human myristoylated alanine rich protein kinase C substrate ELISA Kit;Human myristoylated alanine-rich C-kinase substrate ELISA Kit;Human myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L) ELISA Kit;Human phosphomyristin ELISA Kit;
  • Catalog
    E025562
  • species
    Human
  • GeneMARCKS
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). No significant cross-reactivity or interference between Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman MARCKS ELISA Kit allows for the in vitro quantitative determination of MARCKS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Myristoylated alanine-rich C-kinase substrate Protein information
  • Uniprot ID MARCS_HUMAN
  • Uniprot AC P29966; E1P560; Q2LA83; Q5TDB7;
  • UniGene Hs.519909; Hs.712658;
  • GeneID 4082
  • KEGG hsa:4082;
  • Human Myristoylated alanine-rich C-kinase substrate Protein SEQUENCE

  • SEQUENCE 332 AA; 31555 MW; 0AB247801EF8FCBF CRC64;

    MGAQFSKTAA KGEAAAERPG EAAVASSPSK ANGQENGHVK VNGDASPAAA

    ESGAKEELQA NGSAPAADKE EPAAAGSGAA SPSAAEKGEP AAAAAPEAGA

    SPVEKEAPAE GEAAEPGSPT AAEGEAASAA SSTSSPKAED GATPSPSNET

    PKKKKKRFSF KKSFKLSGFS FKKNKKEAGE GGEAEAPAAE GGKDEAAGGA

    AAAAAEAGAA SGEQAAAPGE EAAAGEEGAA GGDPQEAKPQ EAAVAPEKPP

    ASDETKAAEE PSKVEEKKAE EAGASAAACE APSAAGPGAP PEQEAAPAEE

    PAAAAASSAC AAPSQEAQPE CSPEAPPAEA AE

  • UCSC uc032xir.2; human.;



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