SpecificityThis assay has high sensitivity and excellent specificity for detection of AchRAb. No significant cross-reactivity or interference between AchRAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AchRAb and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman AChR ELISA Kit allows for the in vitro quantitative determination of AChR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyHuman ELISA Kit
Product Description specificalIntended Uses||This AchRAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AchRAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: AchRAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti Acetylcholine Receptor antigen and an AchRAb-HRP conjugate. The assay sample and buffer are incubated together with AchRAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AchRAb concentration since AchRAb from samples and AchRAb-HRP conjugate compete for the Anti Acetylcholine Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by AchRAb from the sample, fewer sites are left to bind AchRAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AchRAb concentration in each sample is interpolated from this standard curve.