Intended UseHuman MYL2 ELISA Kit allows for the in vitro quantitative determination of MYL2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntended Uses||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MYL2 in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MYL2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MYL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MYL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform Protein information
Human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform Protein SEQUENCE