Human MYH7B ELISA Kit

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  • Alternative name

    Myosin-7B ELISA KIT; Antigen MLAA-21 ELISA KIT; Myosin cardiac muscle beta chain ELISA KIT; Myosin heavy chain 7B, cardiac muscle beta isoform ELISA KIT; Slow A MYH14MYH7B ELISA KIT; KIAA1512 ELISA KIT

  • Catalog
    E025437
  • species
    Human
  • GeneMYH7B
  • Standard CurveHuman MYH7B ELISA Kit
  • Other Species Mouse MYH7B ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B). No significant cross-reactivity or interference between Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.069ng/mL.
  • Intended UseHuman MYH7B ELISA Kit allows for the in vitro quantitative determination of MYH7B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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