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Human MYH4 ELISA Kit

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  • Alternative name

    Human myosin heavy chain 2b ELISA Kit;Human MyHC-2B ELISA Kit;Human myosin heavy chain 4 ELISA Kit;Human myosin heavy chain IIb ELISA Kit;Human MyHC-IIb ELISA Kit;Human myosin heavy chain, skeletal muscle, fetal ELISA Kit;Human MYH2B ELISA Kit;Human myosin-4 ELISA Kit;Human myosin, heavy chain 4, skeletal muscle ELISA Kit;Human myosin, heavy polypeptide 4, skeletal muscle ELISA Kit;
  • Catalog
    E025408
  • species
    Human
  • GeneMYH4
  • Standard CurveHuman MYH4 ELISA Kit
  • Other Species Mouse Myh4 ELISA KitCanine MYH4 ELISA KitPorcine MYH4 ELISA KitRabbit MYH4 ELISA KitRat Myh4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MYH4. No significant cross-reactivity or interference between MYH4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYH4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL
  • Intended UseHuman MYH4 ELISA Kit allows for the in vitro quantitative determination of MYH4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This MYH4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MYH4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MYH4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-MYH4 antibody and an MYH4-HRP conjugate. The assay sample and buffer are incubated together with MYH4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYH4 concentration since MYH4 from samples and MYH4-HRP conjugate compete for the anti-MYH4 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYH4 from the sample, fewer sites are left to bind MYH4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYH4 concentration in each sample is interpolated from this standard curve.
  • Human Myosin-4 Protein information
  • Uniprot ID MYH4_HUMAN
  • Uniprot AC Q9Y623;
  • UniGene Hs.711141;
  • GeneID 4622
  • KEGG hsa:4622;
  • Human Myosin-4 Protein SEQUENCE

  • SEQUENCE 1939 AA; 223071 MW; 023D6F73C476B19B CRC64;

    MSSDSEMAIF GEAAPFLRKS EKERIEAQNK PFDAKTSVFV VDPKESYVKA

    IVQSREGGKV TAKTEAGATV TVKEDQVFSM NPPKYDKIED MAMMTHLHEP

    AVLYNLKERY AAWMIYTYSG LFCVTVNPYK WLPVYNPEVV TAYRGKKRQE

    APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA

    VTGEKKKEEP ASGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF

    IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QILSNKKPEL

    IEMLLITTNP YDFAFVSQGE ITVPSIDDQE ELMATDSAVD ILGFTADEKV

    AIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK

    SLCYPRVKVG NEFVTKGQTV QQVYNAVGAL AKAIYEKMFL WMVTRINQQL

    DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC INFTNEKLQQ FFNHHMFVLE

    QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS

    FKNKLYEQHL GKSNNFQKPK PAKGKPEAHF SLVHYAGTVD YNIAGWLDKN

    KDPLNETVVG LYQKSAMKTL AFLFSGAQTA EAEGGGGKKG GKKKGSSFQT

    VSALFRENLN KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN

    GVLEGIRICR KGFPSRILYA DFKQRYKVLN ASAIPEGQFI DSKKASEKLL

    GSIEIDHTQY KFGHTKVFFK AGLLGTLEEM RDEKLAQLIT RTQAICRGFL

    MRVEFRKMME RRESIFCIQY NIRAFMNVKH WPWMKLYFKI KPLLKSAETE

    KEMANMKEEF EKTKEELAKT EAKRKELEEK MVTLMQEKND LQLQVQAEAD

    ALADAEERCD QLIKTKIQLE AKIKEVTERA EDEEEINAEL TAKKRKLEDE

    CSELKKDIDD LELTLAKVEK EKHATENKVK NLTEEMAGLD ETIAKLTKEK

    KALQEAHQQT LDDLQMEEDK VNTLTKAKTK LEQQVDDLEG SLEQEKKLCM

    DLERAKRKLE GDLKLAQEST MDTENDKQQL NEKLKKKEFE MSNLQGKIED

    EQALAIQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR SDLSRELEEI

    SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEESTLQHE ATAAALRKKH

    ADSVAELGEQ IDSLQRVKQK LEKEKSELKM EINDLASNME TVSKAKANFE

    KMCRTLEDQL SEIKTKEEEQ QRLINELSAQ KARLHTESGE FSRQLDEKDA

    MVSQLSRGKQ AFTQQIEELK RQLEEETKAK STLAHALQSA RHDCDLLREQ

    YEEEQEAKAE LQRGMSKANS EVAQWRTKYE TDAIQRTEEL EEAKKKLAQR

    LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN AACIALDKKQ

    RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDHLE

    TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKVKKQLDHE KSELQTSLEE

    AEASLEHEEG KILRIQLELN QVKSEIDRKI AEKDEELDQL KRNHLRVVES

    MQSTLDAEIR SRNDALRIKK KMEGDLNEME IQLNHANRQA AEALRNLRNT

    QGILKDTQLH LDDAIRGQDD LKEQLAMVER RANLMQAEVE ELRASLERTE

    RGRKMAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ IQGEMEDIVQ

    EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQLR

    LDEAEQLALK GGKKQIQKLE ARVRELESEV ESEQKHNVEA VKGLRKHERR

    VKELTYQTEE DRKNILRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF

    RKLQHELEEA KERADIAESQ VNKLRVKSRE VHTKVISEE

  • UCSC uc002gmn.4; human.;


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