Human MB ELISA Kit

Alternative name
Human PVALB ELISA Kit;Human myoglobgin ELISA Kit;Human myoglobin ELISA Kit;
Catalog
E025383
species
Human
GeneMB
SpecificityThis assay has high sensitivity and excellent specificity for detection of human MYO/MB. No significant cross-reactivity or interference between human MYO/MB and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MYO/MB is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman MB ELISA Kit allows for the in vitro quantitative determination of MB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MYO/MB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO/MB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO/MB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO/MB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Myoglobin Protein information
Uniprot ID MYG_HUMAN
Uniprot AC P02144; Q52H51; Q5THY7;
UniGene Hs.517586;
GeneID 4151
KEGG hsa:4151;
Human Myoglobin Protein SEQUENCE
SEQUENCE 154 AA; 17184 MW; F6A41F19A525F09C CRC64;
MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL
KSEDEMKASE DLKKHGATVL TALGGILKKK GHHEAEIKPL AQSHATKHKI
PVKYLEFISE CIIQVLQSKH PGDFGADAQG AMNKALELFR KDMASNYKEL
GFQG
UCSC uc003anz.4; human.;

Human MB ELISA Kit

Human PVALB ELISA Kit;Human myoglobgin ELISA Kit;Human myoglobin ELISA Kit;

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