SensitivityThe minimum detectable dose of human MYO/MB is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman MB ELISA Kit allows for the in vitro quantitative determination of MB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MYO/MB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO/MB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO/MB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO/MB bound in the initial step. The color development is stopped and the intensity of the color is measured.