Human MYF5 ELISA Kit

Alternative name
Human myf-5 ELISA Kit;Human class C basic helix-loop-helix protein 2 ELISA Kit;Human bHLHc2 ELISA Kit;Human myogenic factor 5 ELISA Kit;
Catalog
E025372
species
Human
GeneMYF5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myogenic Factor 5 (MYF5). No significant cross-reactivity or interference between Myogenic Factor 5 (MYF5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.066ng/mL.
Intended UseHuman MYF5 ELISA Kit allows for the in vitro quantitative determination of MYF5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myogenic Factor 5 (MYF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myogenic Factor 5 (MYF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myogenic Factor 5 (MYF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myogenic Factor 5 (MYF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Myogenic factor 5 Protein information
Uniprot ID MYF5_HUMAN
Uniprot AC P13349; Q6ISR9;
UniGene Hs.178023;
GeneID 4617
KEGG hsa:4617;
Human Myogenic factor 5 Protein SEQUENCE
SEQUENCE 255 AA; 28296 MW; 62F00454C729BE66 CRC64;
MDVMDGCQFS PSEYFYDGSC IPSPEGEFGD EFVPRVAAFG AHKAELQGSD
EDEHVRAPTG HHQAGHCLMW ACKACKRKST TMDRRKAATM RERRRLKKVN
QAFETLKRCT TTNPNQRLPK VEILRNAIRY IESLQELLRE QVENYYSLPG
QSCSEPTSPT SNCSDGMPEC NSPVWSRKSS TFDSIYCPDV SNVYATDKNS
LSSLDCLSNI VDRITSSEQP GLPLQDLASL SPVASTDSQP ATPGASSSRL
IYHVL
UCSC uc001szg.3; human.;

Category

Human MYF5 ELISA Kit

Human myf-5 ELISA Kit;Human class C basic helix-loop-helix protein 2 ELISA Kit;Human bHLHc2 ELISA Kit;Human myogenic factor 5 ELISA Kit;