Human MYF5 ELISA Kit

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  • Alternative name

    Human myf-5 ELISA Kit;Human class C basic helix-loop-helix protein 2 ELISA Kit;Human bHLHc2 ELISA Kit;Human myogenic factor 5 ELISA Kit;

  • Catalog
    E025372
  • species
    Human
  • GeneMYF5
  • Standard CurveHuman MYF5 ELISA Kit
  • Other Species Mouse Myf5 ELISA KitBovine MYF5 ELISA KitChicken MYF5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Myogenic Factor 5 (MYF5). No significant cross-reactivity or interference between Myogenic Factor 5 (MYF5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman MYF5 ELISA Kit allows for the in vitro quantitative determination of MYF5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myogenic Factor 5 (MYF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myogenic Factor 5 (MYF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myogenic Factor 5 (MYF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myogenic Factor 5 (MYF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Myogenic factor 5 Protein information
  • Uniprot ID MYF5_HUMAN
  • Uniprot AC P13349; Q6ISR9;
  • UniGene Hs.178023;
  • GeneID 4617
  • KEGG hsa:4617;
  • Human Myogenic factor 5 Protein SEQUENCE
  • SEQUENCE 255 AA; 28296 MW; 62F00454C729BE66 CRC64;

    MDVMDGCQFS PSEYFYDGSC IPSPEGEFGD EFVPRVAAFG AHKAELQGSD

    EDEHVRAPTG HHQAGHCLMW ACKACKRKST TMDRRKAATM RERRRLKKVN

    QAFETLKRCT TTNPNQRLPK VEILRNAIRY IESLQELLRE QVENYYSLPG

    QSCSEPTSPT SNCSDGMPEC NSPVWSRKSS TFDSIYCPDV SNVYATDKNS

    LSSLDCLSNI VDRITSSEQP GLPLQDLASL SPVASTDSQP ATPGASSSRL

    IYHVL

  • UCSC uc001szg.3; human.;




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