Human MEF2C ELISA Kit

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  • Alternative name

    Human C5DELq14.3 ELISA Kit;Human DEL5q14.3 ELISA Kit;Human myocyte enhancer factor 2C ELISA Kit;Human myocyte-specific enhancer factor 2C ELISA Kit;Human MADS box transcription enhancer factor 2, polypeptide C ELISA Kit;

  • Catalog
    E025362
  • species
    Human
  • GeneMEF2C
  • Standard CurveHuman MEF2C ELISA Kit
  • Other Species Mouse Mef2c ELISA KitBovine MEF2C ELISA KitPorcine MEF2C ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MEF2C. No significant cross-reactivity or interference between MEF2C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MEF2C and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MEF2C ELISA Kit allows for the in vitro quantitative determination of MEF2C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This MEF2C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MEF2C. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||MEF2C ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-MEF2C antibody and an MEF2C-HRP conjugate. The assay sample and buffer are incubated together with MEF2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MEF2C concentration since MEF2C from samples and MEF2C-HRP conjugate compete for the anti-MEF2C antibody binding site. Since the number of sites is limited, as more sites are occupied by MEF2C from the sample, fewer sites are left to bind MEF2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MEF2C concentration in each sample is interpolated from this standard curve.
  • Human Myocyte-specific enhancer factor 2C Protein information
  • Uniprot ID MEF2C_HUMAN
  • Uniprot AC Q06413; C9JMZ0; D7F7N5; F8W7V7;
  • UniGene Hs.649965;
  • GeneID 4208
  • KEGG hsa:4208;
  • Human Myocyte-specific enhancer factor 2C Protein SEQUENCE
  • SEQUENCE 473 AA; 51221 MW; A7982020BB8C8949 CRC64;

    MGRKKIQITR IMDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS

    TNKLFQYAST DMDKVLLKYT EYNEPHESRT NSDIVETLRK KGLNGCDSPD

    PDADDSVGHS PESEDKYRKI NEDIDLMISR QRLCAVPPPN FEMPVSIPVS

    SHNSLVYSNP VSSLGNPNLL PLAHPSLQRN SMSPGVTHRP PSAGNTGGLM

    GGDLTSGAGT SAGNGYGNPR NSPGLLVSPG NLNKNMQAKS PPPMNLGMNN

    RKPDLRVLIP PGSKNTMPSV SEDVDLLLNQ RINNSQSAQS LATPVVSVAT

    PTLPGQGMGG YPSAISTTYG TEYSLSSADL SSLSGFNTAS ALHLGSVTGW

    QQQHLHNMPP SALSQLGACT STHLSQSSNL SLPSTQSLNI KSEPVSPPRD

    RTTTPSRYPQ HTRHEAGRSP VDSLSSCSSS YDGSDREDHR NEFHSPIGLT

    RPSPDERESP SVKRMRLSEG WAT

  • UCSC uc003kjj.4; human. [Q06413-1];



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