Human MLH1 ELISA Kit

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  • Alternative name

    Human MutL protein homolog 1 ELISA Kit;Human COCA2 ELISA Kit;Human FCC2 ELISA Kit;Human HNPCC ELISA Kit;Human HNPCC2 ELISA Kit;Human hMLH1 ELISA Kit;Human mutL homolog 1 ELISA Kit;Human DNA mismatch repair protein Mlh1 ELISA Kit;Human mutL homolog 1, colon cancer, nonpolyposis type 2 ELISA Kit;

  • Catalog
    E025199
  • species
    Human
  • GeneMLH1
  • Standard CurveHuman MLH1 ELISA Kit
  • Other Species Mouse Mlh1 ELISA KitRat Mlh1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MLH1). No significant cross-reactivity or interference between MLH1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MLH1 is typically less than 0.053ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
  • Intended UseHuman MLH1 ELISA Kit allows for the in vitro quantitative determination of MLH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MLH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MLH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MLH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MLH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human DNA mismatch repair protein Mlh1 Protein information
  • Uniprot ID MLH1_HUMAN
  • Uniprot AC P40692; B4DI13; B4DQ11; E9PCU2;
  • UniGene Hs.195364;
  • GeneID 4292
  • KEGG hsa:4292;
  • Human DNA mismatch repair protein Mlh1 Protein SEQUENCE
  • SEQUENCE 756 AA; 84601 MW; C9231FC406C2CA20 CRC64;

    MSFVAGVIRR LDETVVNRIA AGEVIQRPAN AIKEMIENCL DAKSTSIQVI

    VKEGGLKLIQ IQDNGTGIRK EDLDIVCERF TTSKLQSFED LASISTYGFR

    GEALASISHV AHVTITTKTA DGKCAYRASY SDGKLKAPPK PCAGNQGTQI

    TVEDLFYNIA TRRKALKNPS EEYGKILEVV GRYSVHNAGI SFSVKKQGET

    VADVRTLPNA STVDNIRSIF GNAVSRELIE IGCEDKTLAF KMNGYISNAN

    YSVKKCIFLL FINHRLVEST SLRKAIETVY AAYLPKNTHP FLYLSLEISP

    QNVDVNVHPT KHEVHFLHEE SILERVQQHI ESKLLGSNSS RMYFTQTLLP

    GLAGPSGEMV KSTTSLTSSS TSGSSDKVYA HQMVRTDSRE QKLDAFLQPL

    SKPLSSQPQA IVTEDKTDIS SGRARQQDEE MLELPAPAEV AAKNQSLEGD

    TTKGTSEMSE KRGPTSSNPR KRHREDSDVE MVEDDSRKEM TAACTPRRRI

    INLTSVLSLQ EEINEQGHEV LREMLHNHSF VGCVNPQWAL AQHQTKLYLL

    NTTKLSEELF YQILIYDFAN FGVLRLSEPA PLFDLAMLAL DSPESGWTEE

    DGPKEGLAEY IVEFLKKKAE MLADYFSLEI DEEGNLIGLP LLIDNYVPPL

    EGLPIFILRL ATEVNWDEEK ECFESLSKEC AMFYSIRKQY ISEESTLSGQ

    QSEVPGSIPN SWKWTVEHIV YKALRSHILP PKHFTEDGNI LQLANLPDLY

    KVFERC

  • UCSC uc003cgl.4; human. [P40692-1];


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