Human MADCAM1 ELISA Kit

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  • Alternative name

    Human MAdCAM-1 ELISA Kit;Human hMAdCAM-1 ELISA Kit;Human MACAM1 ELISA Kit;Human mucosal vascular addressin cell adhesion molecule 1 ELISA Kit;Human mucosal addressin cell adhesion molecule 1 ELISA Kit;

  • Catalog
    E025107
  • species
    Human
  • GeneMADCAM1
  • Standard CurveHuman MADCAM1 ELISA Kit
  • Other Species Mouse Madcam1 ELISA KitRat Madcam1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1). No significant cross-reactivity or interference between Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 37pg/mL.
  • Intended UseHuman MADCAM1 ELISA Kit allows for the in vitro quantitative determination of MADCAM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Mucosal addressin cell adhesion molecule 1 Protein information
  • Uniprot ID MADCA_HUMAN
  • Uniprot AC Q13477; A5PKV4; B2RPL9; O60222; O75867; Q5UGI7;
  • UniGene Hs.102598;
  • GeneID 8174
  • KEGG hsa:8174;
  • Human Mucosal addressin cell adhesion molecule 1 Protein SEQUENCE
  • SEQUENCE 382 AA; 40155 MW; 9DCA60C30BA61E62 CRC64;

    MDFGLALLLA GLLGLLLGQS LQVKPLQVEP PEPVVAVALG ASRQLTCRLA

    CADRGASVQW RGLDTSLGAV QSDTGRSVLT VRNASLSAAG TRVCVGSCGG

    RTFQHTVQLL VYAFPDQLTV SPAALVPGDP EVACTAHKVT PVDPNALSFS

    LLVGGQELEG AQALGPEVQE EEEEPQGDED VLFRVTERWR LPPLGTPVPP

    ALYCQATMRL PGLELSHRQA IPVLHSPTSP EPPDTTSPES PDTTSPESPD

    TTSQEPPDTT SPEPPDKTSP EPAPQQGSTH TPRSPGSTRT RRPEISQAGP

    TQGEVIPTGS SKPAGDQLPA ALWTSSAVLG LLLLALPTYH LWKRCRHLAE

    DDTHPPASLR LLPQVSAWAG LRGTGQVGIS PS

  • UCSC uc002los.4; human. [Q13477-1];



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