Human MUC5AC ELISA Kit

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  • Alternative name

    Human MUC-5AC ELISA Kit;Human gastric mucin ELISA Kit;Human lewis B blood group antigen ELISA Kit;Human leB ELISA Kit;Human major airway glycoprotein ELISA Kit;Human mucin-5 subtype AC, tracheobronchial ELISA Kit;Human tracheobronchial mucin ELISA Kit;Human TBM ELISA Kit;Human MUC5 ELISA Kit;Human mucin ELISA Kit;Human mucin 5AC, oligomeric mucus/gel-forming ELISA Kit;Human mucin-5AC ELISA Kit;Human mucin 5, subtypes A and C, tracheobronchial/gastric ELISA Kit;Human mucin 5AC, oligomeric mucus/gel-forming pseudogene ELISA Kit;

  • Catalog
    E025050
  • species
    Human
  • GeneMUC5AC
  • Standard CurveHuman MUC5AC ELISA Kit
  • Other Species Mouse MUC5AC ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MUC5AC ELISA Kit allows for the in vitro quantitative determination of MUC5AC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: MUC5AC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MUC5AC antibody and an MUC5AC-HRP conjugate. The assay sample and buffer are incubated together with MUC5AC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC5AC concentration since MUC5AC from samples and MUC5AC-HRP conjugate compete for the anti-MUC5AC antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC5AC from the sample, fewer sites are left to bind MUC5AC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC5AC concentration in each sample is interpolated from this standard curve.
  • Human Mucin-5AC Protein information
  • Uniprot ID MUC5A_HUMAN
  • Uniprot AC HRPHPTPT TVGPTTVGST TVGPTTVGST TVGPTTPPAP CLPSPICQLI LSKVFEPCHT
  • UniGene Hs.534332; Hs.558950; Hs.703588; Hs.703728; Hs.721515;
  • GeneID 4586
  • KEGG hsa:4586;
  • Human Mucin-5AC Protein SEQUENCE
  • SEQUENCE 5654 AA; 585570 MW; 13217A8257E8E2DE CRC64;

    MSVGRRKLAL LWALALALAC TRHTGHAQDG SSESSYKHHP ALSPIARGPS

    GVPLRGATVF PSLRTIPVVR ASNPAHNGRV CSTWGSFHYK TFDGDVFRFP

    GLCNYVFSEH CGAAYEDFNI QLRRSQESAA PTLSRVLMKV DGVVIQLTKG

    SVLVNGHPVL LPFSQSGVLI QQSSSYTKVE ARLGLVLMWN HDDSLLLELD

    TKYANKTCGL CGDFNGMPVV SELLSHNTKL TPMEFGNLQK MDDPTDQCQD

    PVPEPPRNCS TGFGICEELL HGQLFSGCVA LVDVGSYLEA CRQDLCFCED

    TDLLSCVCHT LAEYSRQCTH AGGLPQDWRG PDFCPQKCPN NMQYHECRSP

    CADTCSNQEH SRACEDHCVA GCFCPEGTVL DDIGQTGCVP VSKCACVYNG

    AAYAPGATYS TDCTNCTCSG GRWSCQEVPC PGTCSVLGGA HFSTFDGKQY

    TVHGDCSYVL TKPCDSSAFT VLAELRRCGL TDSETCLKSV TLSLDGAQTV

    VVIKASGEVF LNQIYTQLPI SAANVTIFRP STFFIIAQTS LGLQLNLQLV

    PTMQLFMQLA PKLRGQTCGL CGNFNSIQAD DFRTLSGVVE ATAAAFFNTF

    KTQAACPNIR NSFEDPCSLS VENEKYAQHW CSQLTDADGP FGRCHAAVKP

    GTYYSNCMFD TCNCERSEDC LCAALSSYVH ACAAKGVQLG GWRDGVCTKP

    MTTCPKSMTY HYHVSTCQPT CRSLSEGDIT CSVGFIPVDG CICPKGTFLD

    DTGKCVQASN CPCYHRGSMI PNGESVHDSG AICTCTHGKL SCIGGQAPAP

    VCAAPMVFFD CRNATPGDTG AGCQKSCHTL DMTCYSPQCV PGCVCPDGLV

    ADGEGGCITA EDCPCVHNEA SYRAGQTIRV GCNTCTCDSR MWRCTDDPCL

    ATCAVYGDGH YLTFDGQSYS FNGDCEYTLV QNHCGGKDST QDSFRVVTEN

    VPCGTTGTTC SKAIKIFLGG FELKLSHGKV EVIGTDESQE VPYTIRQMGI

    YLVVDTDIGL VLLWDKKTSI FINLSPEFKG RVCGLCGNFD DIAVNDFATR

    SRSVVGDVLE FGNSWKLSPS CPDALAPKDP CTANPFRKSW AQKQCSILHG

    PTFAACHAHV EPARYYEACV NDACACDSGG DCECFCTAVA AYAQACHEVG

    LCVSWRTPSI CPLFCDYYNP EGQCEWHYQP CGVPCLRTCR NPRGDCLRDV

    RGLEGCYPKC PPEAPIFDED KMQCVATCPT PPLPPRCHVH GKSYRPGAVV

    PSDKNCQSCL CTERGVECTY KAEACVCTYN GQRFHPGDVI YHTTDGTGGC

    ISARCGANGT IERRVYPCSP TTPVPPTTFS FSTPPLVVSS THTPSNGPSS

    AHTGPPSSAW PTTAGTSPRT RLPTASASLP PVCGEKCLWS PWMDVSRPGR

    GTDSGDFDTL ENLRAHGYRV CESPRSVECR AEDAPGVPLR ALGQRVQCSP

    DVGLTCRNRE QASGLCYNYQ IRVQCCTPLP CSTSSSPAQT TPPTTSKTTE

    TRASGSSAPS STPGTVSLST ARTTPAPGTA TSVKKTFSTP SPPPVPATST

    SSMSTTAPGT SVVSSKPTPT EPSTSSCLQE LCTWTEWIDG SYPAPGINGG

    DFDTFQNLRD EGYTFCESPR SVQCRAESFP NTPLADLGQD VICSHTEGLI

    CLNKNQLPPI CYNYEIRIQC CETVNVCRDI TRLPKTVATT RPTPHPTGAQ

    TQTTFTTHMP SASTEQPTAT SRGGPTATSV TQGTHTTLVT RNCHPRCTWT

    KWFDVDFPSP GPHGGDKETY NNIIRSGEKI CRRPEEITRL QCRAKSHPEV

    SIEHLGQVVQ CSREEGLVCR NQDQQGPFKM CLNYEVRVLC CETPRGCHMT

    STPGSTSSSP AQTTPSTTSK TTETQASGSS APSSTPGTVS LSTARTTPAP

    GTATSVKKTF STPSPPPVPA TSTSSMSTTA PGTSVVSSKP TPTEPSTSSC

    LQELCTWTEW IDGSYPAPGI NGGDFDTFQN LRDEGYTFCE SPRSVQCRAE

    SFPNTPLADL GQDVICSHTE GLICLNKNQL PPICYNYEIR IQCCETVNVC

    RDITRPPKTV ATTRPTPHPT GAQTQTTFTT HMPSASTEQP TATSRGGPTA

    TSVTQGTHTT PVTRNCHPRC TWTTWFDVDF PSPGPHGGDK ETYNNIIRSG

    EKICRRPEEI TRLQCRAKSH PEVSIEHLGQ VVQCSREEGL VCRNQDQQGP

    FKMCLNYEVR VLCCETPKGC PVTSTPVTAP STPSGRATSP TQSTSSWQKS

    RTTTLVTTST TSTPQTSTTY AHTTSTTSAP TARTTSAPTT RTTSASPAST

    TSGPGNTPSP VPTTSTISAP TTSITSAPTT STTSAPTSST TSGPGTTPSP

    VPTTSITSAP TTSTTSAPTT STTSARTSST TSATTTSRIS GPETTPSPVP

    TTSTTSATTT STTSAPTTST TSAPTSSTTS SPQTSTTSAP TTSTTSGPGT

    TPSPVPTTST TSAPTTRTTS APKSSTTSAA TTSTTSGPET TPRPVPTTST

    TSSPTTSTTS APTTSTTSAS TTSTTSGAGT TPSPVPTTST TSAPTTSTTS

    APISSTTSAT TTSTTSGPGT TPSPVPTTST TSAPTTSTTS GPGTTPSAVP

    TTSITSAPTT STNSAPISST TSATTTSRIS GPETTPSPVP TASTTSASTT

    STTSGPGTTP SPVPTTSTIS VPTTSTTSAS TTSTTSASTT STTSGPGTTP

    SPVPTTSTTS APTTSTTSAP TTSTISAPTT STTSATTTST TSAPTPRRTS

    APTTSTISAS TTSTTSATTT STTSATTTST ISAPTTSTTL SPTTSTTSTT

    ITSTTSAPIS STTSTPQTST TSAPTTSTTS GPGTTSSPVP TTSTTSAPTT

    STTSAPTTRT TSVPTSSTTS TATTSTTSGP GTTPSPVPTT STTSAPTTRT

    TSAPTTSTTS APTTSTTSAP TSSTTSATTT STISVPTTST TSVPGTTPSP

    VPTTSTISVP TTSTTSASTT STTSGPGTTP SPVPTTSTTS APTTSTTSAP

    TTSTISAPTT STPSAPTTST TLAPTTSTTS APTTSTTSTP TSSTTSSPQT

    STTSASTTSI TSGPGTTPSP VPTTSTTSAP TTSTTSAATT STISAPTTST

    TSAPTTSTTS ASTASKTSGL GTTPSPIPTT STTSPPTTST TSASTASKTS

    GPGTTPSPVP TTSTIFAPRT STTSASTTST TPGPGTTPSP VPTTSTASVS

    KTSTSHVSIS KTTHSQPVTR DCHLRCTWTK WFDIDFPSPG PHGGDKETYN

    NIIRSGEKIC RRPEEITRLQ CRAESHPEVS IEHLGQVVQC SREEGLVCRN

    QDQQGPFKMC LNYEVRVLCC ETPKGCPVTS TPVTAPSTPS GRATSPTQST

    SSWQKSRTTT LVTTSTTSTP QTSTTSAPTT STTSAPTTST TSAPTTSTTS

    TPQTSISSAP TSSTTSAPTS STISARTTSI ISAPTTSTTS SPTTSTTSAT

    TTSTTSAPTS STTSTPQTSK TSAATSSTTS GSGTTPSPVT TTSTASVSKT

    STSHVSVSKT THSQPVTRDC HPRCTWTKWF DVDFPSPGPH GGDKETYNNI

    IRSGEKICRR PEEITRLQCR AKSHPEVSIE HLGQVVQCSR EEGLVCRNQD

    QQGPFKMCLN YEVRVLCCET PKGCPVTSTS VTAPSTPSGR ATSPTQSTSS

    WQKSRTTTLV TSSITSTTQT STTSAPTTST TPASIPSTTS APTTSTTSAP

    TTSTTSAPTT STTSTPQTTT SSAPTSSTTS APTTSTISAP TTSTISAPTT

    STTSAPTAST TSAPTSTSSA PTTNTTSAPT TSTTSAPITS TISAPTTSTT

    STPQTSTISS PTTSTTSTPQ TSTTSSPTTS TTSAPTTSTT SAPTTSTTST

    PQTSISSAPT SSTTSAPTAS TISAPTTSTT SFHTTSTTSP PTSSTSSTPQ

    TSKTSAATSS TTSGSGTTPS PVPTTSTASV SKTSTSHVSV SKTTHSQPVT

    RDCHPRCTWT KWFDVDFPSP GPHGGDKETY NNIIRSGEKI CRRPEEITRL

    QCRAESHPEV SIEHLGQVVQ CSREEGLVCR NQDQQGPFKM CLNYEVRVLC

    CETPKGCPVT STPVTAPSTP SGRATSPTQS TSSWQKSRTT TLVTTSTTST

    PQTSTTSAPT TSTIPASTPS TTSAPTTSTT SAPTTSTTSA PTHRTTSGPT

    TSTTLAPTTS TTSAPTTSTN SAPTTSTISA STTSTISAPT TSTISSPTSS

    TTSTPQTSKT SAATSSTTSG SGTTPSPVPT TSTTSASTTS TTSAPTTSTT

    SGPGTTPSPV PSTSTTSAAT TSTTSAPTTR TTSAPTSSMT SGPGTTPSPV

    PTTSTTSAPT TSTTSGPGTT PSPVPTTSTT SAPITSTTSG PGSTPSPVPT

    TSTTSAPTTS TTSASTASTT SGPGTTPSPV PTTSTTSAPT TRTTSASTAS

    TTSGPGSTPS PVPTTSTTSA PTTRTTPAST ASTTSGPGTT PSPVPTTSTT

    SASTTSTISL PTTSTTSAPI TSMTSGPGTT PSPVPTTSTT SAPTTSTTSA

    STASTTSGPG TTPSPVPTTS TTSAPTTSTT SASTASTTSG PGTSLSPVPT

    TSTTSAPTTS TTSGPGTTPS PVPTTSTTSA PTTSTTSGPG TTPSPVPTTS

    TTPVSKTSTS HLSVSKTTHS QPVTSDCHPL CAWTKWFDVD FPSPGPHGGD

    KETYNNIIRS GEKICRRPEE ITRLQCRAES HPEVNIEHLG QVVQCSREEG

    LVCRNQDQQG PFKMCLNYEV RVLCCETPRG CPVTSVTPYG TSPTNALYPS

    LSTSMVSASV ASTSVASSSV ASSSVAYSTQ TCFCNVADRL YPAGSTIYRH

    RDLAGHCYYA LCSQDCQVVR GVDSDCPSTT LPPAPATSPS ISTSEPVTEL

    GCPNAVPPRK KGETWATPNC SEATCEGNNV ISLRPRTCPR VEKPTCANGY

    PAVKVADQDG CCHHYQCQCV CSGWGDPHYI TFDGTYYTFL DNCTYVLVQQ

    IVPVYGHFRV LVDNYFCGAE DGLSCPRSII LEYHQDRVVL TRKPVHGVMT

    NEIIFNNKVV SPGFRKNGIV VSRIGVKMYA TIPELGVQVM FSGLIFSVEV

    PFSKFANNTE GQCGTCTNDR KDECRTPRGT VVASCSEMSG LWNVSIPDQP

    ACHRPHPTPT TVGPTTVGST TVGPTTVGST TVGPTTPPAP CLPSPICQLI

    LSKVFEPCHT VIPPLLFYEG CVFDRCHMTD LDVVCSSLEL YAALCASHDI

    CIDWRGRTGH MCPFTCPADK VYQPCGPSNP SYCYGNDSAS LGALPEAGPI

    TEGCFCPEGM TLFSTSAQVC VPTGCPRCLG PHGEPVKVGH TVGMDCQECT

    CEAATWTLTC RPKLCPLPPA CPLPGFVPVP AAPQAGQCCP QYSCACNTSR

    CPAPVGCPEG ARAIPTYQEG ACCPVQNCSW TVCSINGTLY QPGAVVSSSL

    CETCRCELPG GPPSDAFVVS CETQICNTHC PVGFEYQEQS GQCCGTCVQV

    ACVTNTSKSP AHLFYPGETW SDAGNHCVTH QCEKHQDGLV VVTTKKACPP

    LSCSLDEARM SKDGCCRFCP PPPPPYQNQS TCAVYHRSLI IQQQGCSSSE

    PVRLAYCRGN CGDSSSMYSL EGNTVEHRCQ CCQELRTSLR NVTLHCTDGS

    SRAFSYTEVE ECGCMGRRCP APGDTQHSEE AEPEPSQEAE SGSWERGVPV

    SPMH

  • UCSC uc031xcx.2; human.;



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