Human MUC1 ELISA Kit

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  • Alternative name

    Human MUC-1 ELISA Kit;Human breast carcinoma-associated antigen DF3 ELISA Kit;Human cancer antigen 15-3 ELISA Kit;Human CA 15-3 ELISA Kit;Human carcinoma-associated mucin ELISA Kit;Human episialin ELISA Kit;Human H23AG ELISA Kit;Human krebs von den Lungen-6 ELISA Kit;Human KL-6 ELISA Kit;Human PEMT ELISA Kit;Human peanut-reactive urinary mucin ELISA Kit;Human PUM ELISA Kit;Human polymorphic epithelial mucin ELISA Kit;Human PEM ELISA Kit;Human tumor-associated epithelial membrane antigen ELISA Kit;Human EMA ELISA Kit;Human Tumor-associated mucin ELISA Kit;Human MUC1-NT ELISA Kit;Human MUC1-alpha ELISA Kit;Human MUC1-beta ELISA Kit;Human MUC1-CT ELISA Kit;Human ADMCKD ELISA Kit;Human ADMCKD1 ELISA Kit;Human CD227 ELISA Kit;Human MAM6 ELISA Kit;Human MCD ELISA Kit;Human MCKD ELISA Kit;Human MCKD1 ELISA Kit;Human MUC-1/SEC ELISA Kit;Human MUC-1/X ELISA Kit;Human MUC1/ZD ELISA Kit;Human mucin 1, cell surface associated ELISA Kit;Human mucin-1 ELISA Kit;Human H23 antigen ELISA Kit;Human mucin 1, transmembrane ELISA Kit;Human tumor associated epithelial mucin ELISA Kit;

  • Catalog
    E025018
  • species
    Human
  • GeneMUC1
  • Standard CurveHuman MUC1 ELISA Kit
  • Other Species Mouse Muc1 ELISA KitBovine MUC1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MUC1. No significant cross-reactivity or interference between MUC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MUC1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MUC1 ELISA Kit allows for the in vitro quantitative determination of MUC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the Assay: MUC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MUC1 antibody and an MUC1-HRP conjugate. The assay sample and buffer are incubated together with MUC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC1 concentration since MUC1 from samples and MUC1-HRP conjugate compete for the anti-MUC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC1 from the sample, fewer sites are left to bind MUC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC1 concentration in each sample is interpolated from this standard curve.
  • Human Mucin-1 subunit beta Protein information
  • Uniprot ID MUC1_HUMAN
  • Uniprot AC Q9BXA4; Q9UE75; Q9UE76; Q9UQL1; Q9Y4J2;
  • UniGene Hs.89603;
  • GeneID 4582
  • KEGG hsa:4582;
  • Human Mucin-1 subunit beta Protein SEQUENCE
  • SEQUENCE 1255 AA; 122102 MW; 5E28DFC4C20D9A82 CRC64;

    MTPGTQSPFF LLLLLTVLTV VTGSGHASST PGGEKETSAT QRSSVPSSTE

    KNAVSMTSSV LSSHSPGSGS STTQGQDVTL APATEPASGS AATWGQDVTS

    VPVTRPALGS TTPPAHDVTS APDNKPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS

    TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS

    APDTRPAPGS TAPPAHGVTS APDTRPAPGS TAPPAHGVTS APDNRPALGS

    TAPPVHNVTS ASGSASGSAS TLVHNGTSAR ATTTPASKST PFSIPSHHSD

    TPTTLASHST KTDASSTHHS SVPPLTSSNH STSPQLSTGV SFFFLSFHIS

    NLQFNSSLED PSTDYYQELQ RDISEMFLQI YKQGGFLGLS NIKFRPGSVV

    VQLTLAFREG TINVHDVETQ FNQYKTEAAS RYNLTISDVS VSDVPFPFSA

    QSGAGVPGWG IALLVLVCVL VALAIVYLIA LAVCQCRRKN YGQLDIFPAR

    DTYHPMSEYP TYHTHGRYVP PSSTDRSPYE KVSAGNGGSS LSYTNPAVAA

    TSANL

  • UCSC uc001fia.4; human. [P15941-1];


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