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Human SMAD2 ELISA Kit

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  • Alternative name

    Human MAD homolog 2 ELISA Kit;Human Mothers against DPP homolog 2 ELISA Kit;Human JV18-1 ELISA Kit;Human Mad-related protein 2 ELISA Kit;Human hMAD-2 ELISA Kit;Human SMAD family member 2 ELISA Kit;Human SMAD 2 ELISA Kit;Human Smad2 ELISA Kit;Human hSMAD2 ELISA Kit;Human MADH2 ELISA Kit;Human MADR2 ELISA Kit;Human JV18 ELISA Kit;Human mothers against decapentaplegic homolog 2 ELISA Kit;Human SMAD, mothers against DPP homolog 2 ELISA Kit;Human Sma- and Mad-related protein 2 ELISA Kit;Human mother against DPP homolog 2 ELISA Kit;
  • Catalog
    E024916
  • species
    Human
  • GeneSMAD2
  • SpecificityThis assay has high sensitivity for detection of Smad2. 100% cross-reactivity of Smad2 was observed among human, mouse and rat.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.059ng/mL.
  • Intended UseHuman SMAD2 ELISA Kit allows for the in vitro quantitative determination of SMAD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Smad2 in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 99% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Smad2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Smad2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Smad2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Smad2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Mothers against decapentaplegic homolog 2 Protein information
  • Uniprot ID SMAD2_HUMAN
  • Uniprot AC Q15796;
  • UniGene Hs.12253; Hs.705764; Hs.741342;
  • GeneID 4087
  • KEGG hsa:4087;
  • Human Mothers against decapentaplegic homolog 2 Protein SEQUENCE

  • SEQUENCE 467 AA; 52306 MW; 95406DB5FC0AA4C9 CRC64;

    MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK

    KLKKTGRLDE LEKAITTQNC NTKCVTIPST CSEIWGLSTP NTIDQWDTTG

    LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE

    YAFNLKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEILTEL PPLDDYTHSI

    PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS

    PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY ELNQRVGETF HASQPSLTVD

    GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL

    SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ

    GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD

    KVLTQMGSPS VRCSSMS

  • UCSC uc010xdc.4; human. [Q15796-1];



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