Human MAOA ELISA Kit

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  • Alternative name

    Human monoamine oxidase type A ELISA Kit;Human MAO-A ELISA Kit;Human BRNRS ELISA Kit;Human monoamine oxidase A ELISA Kit;Human amine oxidase [flavin-containing] A ELISA Kit;

  • Catalog
    E024852
  • species
    Human
  • GeneMAOA
  • Standard CurveHuman MAOA ELISA Kit
  • Other Species Mouse Maoa ELISA KitBovine MAOA ELISA KitCanine MAOA ELISA KitHorse MAOA ELISA KitPorcine MAOA ELISA KitRat Maoa ELISA KitSheep MAOA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MOA. No significant cross-reactivity or interference between MOA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MOA and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MAOA ELISA Kit allows for the in vitro quantitative determination of MAOA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: MOA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MOA antibody and an MOA-HRP conjugate. The assay sample and buffer are incubated together with MOA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MOA concentration since MOA from samples and MOA-HRP conjugate compete for the anti-MOA antibody binding site. Since the number of sites is limited, as more sites are occupied by MOA from the sample, fewer sites are left to bind MOA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MOA concentration in each sample is interpolated from this standard curve.
  • Human Amine oxidase [flavin-containing] A Protein information
  • Uniprot ID AOFA_HUMAN
  • Uniprot AC P21397; B4DF46; Q16426;
  • UniGene Hs.183109;
  • GeneID 4128
  • KEGG hsa:4128;
  • Human Amine oxidase [flavin-containing] A Protein SEQUENCE
  • SEQUENCE 527 AA; 59682 MW; 4270E346928AE832 CRC64;

    MENQEKASIA GHMFDVVVIG GGISGLSAAK LLTEYGVSVL VLEARDRVGG

    RTYTIRNEHV DYVDVGGAYV GPTQNRILRL SKELGIETYK VNVSERLVQY

    VKGKTYPFRG AFPPVWNPIA YLDYNNLWRT IDNMGKEIPT DAPWEAQHAD

    KWDKMTMKEL IDKICWTKTA RRFAYLFVNI NVTSEPHEVS ALWFLWYVKQ

    CGGTTRIFSV TNGGQERKFV GGSGQVSERI MDLLGDQVKL NHPVTHVDQS

    SDNIIIETLN HEHYECKYVI NAIPPTLTAK IHFRPELPAE RNQLIQRLPM

    GAVIKCMMYY KEAFWKKKDY CGCMIIEDED APISITLDDT KPDGSLPAIM

    GFILARKADR LAKLHKEIRK KKICELYAKV LGSQEALHPV HYEEKNWCEE

    QYSGGCYTAY FPPGIMTQYG RVIRQPVGRI FFAGTETATK WSGYMEGAVE

    AGERAAREVL NGLGKVTEKD IWVQEPESKD VPAVEITHTF WERNLPSVSG

    LLKIIGFSTS VTALGFVLYK YKLLPRS

  • UCSC uc011mkw.3; human. [P21397-1];


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