Human MSN ELISA Kit

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  • Alternative name

    Human membrane-organizing extension spike protein ELISA Kit;Human HEL70 ELISA Kit;Human IMD50 ELISA Kit;Human moesin ELISA Kit;Human epididymis luminal protein 70 ELISA Kit;

  • Catalog
    E024830
  • species
    Human
  • GeneMSN
  • Standard CurveHuman MSN ELISA Kit
  • Other Species Mouse Msn ELISA KitBovine MSN ELISA KitPorcine MSN ELISA KitRat Msn ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MSN. No significant cross-reactivity or interference between MSN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MSN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MSN ELISA Kit allows for the in vitro quantitative determination of MSN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This MSN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MSN. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||MSN ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-MSN antibody and an MSN-HRP conjugate. The assay sample and buffer are incubated together with MSN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MSN concentration since MSN from samples and MSN-HRP conjugate compete for the anti-MSN antibody binding site. Since the number of sites is limited, as more sites are occupied by MSN from the sample, fewer sites are left to bind MSN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSN concentration in each sample is interpolated from this standard curve.
  • Human Moesin Protein information
  • Uniprot ID MOES_HUMAN
  • Uniprot AC P26038;
  • UniGene Hs.713679; Hs.87752;
  • GeneID 4478
  • KEGG hsa:4478;
  • Human Moesin Protein SEQUENCE
  • SEQUENCE 577 AA; 67820 MW; 865A6C5CB14AE586 CRC64;

    MPKTISVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWFFGLQYQ

    DTKGFSTWLK LNKKVTAQDV RKESPLLFKF RAKFYPEDVS EELIQDITQR

    LFFLQVKEGI LNDDIYCPPE TAVLLASYAV QSKYGDFNKE VHKSGYLAGD

    KLLPQRVLEQ HKLNKDQWEE RIQVWHEEHR GMLREDAVLE YLKIAQDLEM

    YGVNYFSIKN KKGSELWLGV DALGLNIYEQ NDRLTPKIGF PWSEIRNISF

    NDKKFVIKPI DKKAPDFVFY APRLRINKRI LALCMGNHEL YMRRRKPDTI

    EVQQMKAQAR EEKHQKQMER AMLENEKKKR EMAEKEKEKI EREKEELMER

    LKQIEEQTKK AQQELEEQTR RALELEQERK RAQSEAEKLA KERQEAEEAK

    EALLQASRDQ KKTQEQLALE MAELTARISQ LEMARQKKES EAVEWQQKAQ

    MVQEDLEKTR AELKTAMSTP HVAEPAENEQ DEQDENGAEA SADLRADAMA

    KDRSEEERTT EAEKNERVQK HLKALTSELA NARDESKKTA NDMIHAENMR

    LGRDKYKTLR QIRQGNTKQR IDEFESM



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