Human MAPK8 ELISA Kit

Alternative name
Human MAP kinase 8 ELISA Kit;Human MAPK 8 ELISA Kit;Human JNK-46 ELISA Kit;Human stress-activated protein kinase 1c ELISA Kit;Human SAPK1c ELISA Kit;Human Stress-activated protein kinase JNK1 ELISA Kit;Human c-Jun N-terminal kinase 1 ELISA Kit;Human JNK1 ELISA Kit;Human PRKM8 ELISA Kit;Human SAPK1 ELISA Kit;Human JNK ELISA Kit;Human JNK1A2 ELISA Kit;Human JNK21B1/2 ELISA Kit;Human mitogen-activated protein kinase 8 ELISA Kit;Human JUN N-terminal kinase ELISA Kit;Human mitogen-activated protein kinase 8 isoform JNK1 alpha1 ELISA Kit;Human mitogen-activated protein kinase 8 isoform JNK1 beta2 ELISA Kit;Human stress-activated protein kinase 1 ELISA Kit;
Catalog
E024732
species
Human
GeneMAPK8
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman MAPK8 ELISA Kit allows for the in vitro quantitative determination of MAPK8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human MAPK8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MAPK8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MAPK8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MAPK8 can be calculated.
Human Mitogen-activated protein kinase 8 Protein information
Uniprot ID MK08_HUMAN
Uniprot AC Q308M2;
UniGene Hs.138211; Hs.522924;
GeneID 5599
KEGG hsa:5599;
Human Mitogen-activated protein kinase 8 Protein SEQUENCE
SEQUENCE 427 AA; 48296 MW; 94FB6BE0358B9B60 CRC64;
MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE
FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR
DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM
GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK
KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE
EWKELIYKEV MDLEERTKNG VIRGQPSPLG AAVINGSQHP SSSSSVNDVS
SMSTDPTLAS DTDSSLEAAA GPLGCCR
UCSC uc001jgm.5; human. [P45983-1];

Category

Human MAPK8 ELISA Kit