Human MAP3K15 ELISA Kit

Contact Us
  • Alternative name

    Human apoptosis signal-regulating kinase 3 ELISA Kit;Human MAPK/ERK kinase kinase 15 ELISA Kit;Human MEK kinase 15 ELISA Kit;Human MEKK 15 ELISA Kit;Human ASK3 ELISA Kit;Human bA723P2.3 ELISA Kit;Human mitogen-activated protein kinase kinase kinase 15 ELISA Kit;

  • Catalog
    E024727
  • species
    Human
  • GeneMAP3K15
  • Standard CurveHuman MAP3K15 ELISA Kit
  • Other Species Mouse Map3k15 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MAP3K15. No significant cross-reactivity or interference between MAP3K15 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAP3K15 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman MAP3K15 ELISA Kit allows for the in vitro quantitative determination of MAP3K15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: MAP3K15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAP3K15 antibody and an MAP3K15-HRP conjugate. The assay sample and buffer are incubated together with MAP3K15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAP3K15 concentration since MAP3K15 from samples and MAP3K15-HRP conjugate compete for the anti-MAP3K15 antibody binding site. Since the number of sites is limited, as more sites are occupied by MAP3K15 from the sample, fewer sites are left to bind MAP3K15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAP3K15 concentration in each sample is interpolated from this standard curve.
  • Human Mitogen-activated protein kinase kinase kinase 15 Protein information
  • Uniprot ID M3K15_HUMAN
  • Uniprot AC Q6ZN16; A2AI49; A2AI50; A6NJ61; Q5JPR4; Q6ZMV3;
  • UniGene Hs.713701;
  • GeneID 389840
  • KEGG hsa:389840;
  • Human Mitogen-activated protein kinase kinase kinase 15 Protein SEQUENCE
  • SEQUENCE 1313 AA; 147437 MW; 60751706D6AEE3E9 CRC64;

    MESGGGNAPA GALGAASESP QCPPPPGVEG AAGPAEPDGA AEGAAGGSGE

    GESGGGPRRA LRAVYVRSES SQGGAAGGPE AGARQCLLRA CEAEGAHLTS

    VPFGELDFGE TAVLDAFYDA DVAVVDMSDV SRQPSLFYHL GVRESFDMAN

    NVILYHDTDA DTALSLKDMV TQKNTASSGN YYFIPYIVTP CADYFCCESD

    AQRRASEYMQ PNWDNILGPL CMPLVDRFIS LLKDIHVTSC VYYKETLLND

    IRKAREKYQG EELAKELARI KLRMDNTEVL TSDIIINLLL SYRDIQDYDA

    MVKLVETLEM LPTCDLADQH NIKFHYAFAL NRRNSTGDRE KALQIMLQVL

    QSCDHPGPDM FCLCGRIYKD IFLDSDCKDD TSRDSAIEWY RKGFELQSSL

    YSGINLAVLL IVAGQQFETS LELRKIGVRL NSLLGRKGSL EKMNNYWDVG

    QFFSVSMLAH DVGKAVQAAE RLFKLKPPVW YLRSLVQNLL LIRRFKKTII

    EHSPRQERLN FWLDIIFEAT NEVTNGLRFP VLVIEPTKVY QPSYVSINNE

    AEERTVSLWH VSPTEMKQMH EWNFTASSIK GISLSKFDER CCFLYVHDNS

    DDFQIYFSTE EQCSRFFSLV KEMITNTAGS TVELEGETDG DTLEYEYDHD

    ANGERVVLGK GTYGIVYAGR DLSNQVRIAI KEIPERDSRY SQPLHEEIAL

    HKYLKHRNIV QYLGSVSENG YIKIFMEQVP GGSLSALLRS KWGPMKEPTI

    KFYTKQILEG LKYLHENQIV HRDIKGDNVL VNTYSGVVKI SDFGTSKRLA

    GVNPCTETFT GTLQYMAPEI IDQGPRGYGA PADIWSLGCT IIEMATSKPP

    FHELGEPQAA MFKVGMFKIH PEIPEALSAE ARAFILSCFE PDPHKRATTA

    ELLREGFLRQ VNKGKKNRIA FKPSEGPRGV VLALPTQGEP MATSSSEHGS

    VSPDSDAQPD ALFERTRAPR HHLGHLLSVP DESSALEDRG LASSPEDRDQ

    GLFLLRKDSE RRAILYKILW EEQNQVASNL QECVAQSSEE LHLSVGHIKQ

    IIGILRDFIR SPEHRVMATT ISKLKVDLDF DSSSISQIHL VLFGFQDAVN

    KILRNHLIRP HWMFAMDNII RRAVQAAVTI LIPELRAHFE PTCETEGVDK

    DMDEAEEGYP PATGPGQEAQ PHQQHLSLQL GELRQETNRL LEHLVEKERE

    YQNLLRQTLE QKTQELYHLQ LKLKSNCITE NPAGPYGQRT DKELIDWLRL

    QGADAKTIEK IVEEGYTLSD ILNEITKEDL RYLRLRGGLL CRLWSAVSQY

    RRAQEASETK DKA

  • UCSC uc004czj.3; human. [Q6ZN16-1];



Inquiry About Human MAP3K15 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!