Human MAPK1 ELISA Kit

Alternative name
Human MAP kinase 1 ELISA Kit;Human MAPK 1 ELISA Kit;Human ERT1 ELISA Kit;Human extracellular signal-regulated kinase 2 ELISA Kit;Human ERK-2 ELISA Kit;Human MAP kinase isoform p42 ELISA Kit;Human p42-MAPK ELISA Kit;Human mitogen-activated protein kinase 2 ELISA Kit;Human MAP kinase 2 ELISA Kit;Human MAPK 2 ELISA Kit;Human ERK2 ELISA Kit;Human PRKM1 ELISA Kit;Human PRKM2 ELISA Kit;Human ERK ELISA Kit;Human MAPK2 ELISA Kit;Human P42MAPK ELISA Kit;Human p38 ELISA Kit;Human p40 ELISA Kit;Human p41 ELISA Kit;Human p41mapk ELISA Kit;Human mitogen-activated protein kinase 1 ELISA Kit;Human protein tyrosine kinase ERK2 ELISA Kit;
Catalog
E024718
species
Human
GeneMAPK1
SpecificityThis assay has high sensitivity and excellent specificity for detection of MAPK1 . No significant cross-reactivity or interference between MAPK1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.094ng/ml
Intended UseHuman MAPK1 ELISA Kit allows for the in vitro quantitative determination of MAPK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MAPK1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MAPK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAPK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAPK1 can be calculated.
Human Mitogen-activated protein kinase 1 Protein information
Uniprot ID MK01_HUMAN
Uniprot AC P28482; A8CZ64;
UniGene Hs.431850;
GeneID 5594
KEGG hsa:5594;
Human Mitogen-activated protein kinase 1 Protein SEQUENCE
SEQUENCE 360 AA; 41390 MW; E85D0B2A5D2D724E CRC64;
MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR
VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD
VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL
KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML
NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE
DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK
RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE
TARFQPGYRS
UCSC uc010gtk.2; human. [P28482-1];

Category

Human MAPK1 ELISA Kit