SensitivityThe minimum detectable dose of human MAPK is typically less than 33 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human MAPK concentration that could be differentiated from zero. It was determined the me
Intended UseHuman MAPK ELISA Kit allows for the in vitro quantitative determination of MAPK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAPK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for MAPK. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK bound in the initial step. The color development is stopped and the intensity of the color is measured.