Human MAPK14 ELISA Kit

Alternative name
Human MAP kinase 14 ELISA Kit;Human MAPK 14 ELISA Kit;Human Cytokine suppressive anti-inflammatory drug-binding protein ELISA Kit;Human CSAID-binding protein ELISA Kit;Human CSBP ELISA Kit;Human MAP kinase Mxi2 ELISA Kit;Human MAX-interacting protein 2 ELISA Kit;Human mitogen-activated protein kinase p38 alpha ELISA Kit;Human MAP kinase p38 alpha ELISA Kit;Human stress-activated protein kinase 2A ELISA Kit;Human SAPK2A ELISA Kit;Human CSBP1 ELISA Kit;Human CSBP2 ELISA Kit;Human CSPB1 ELISA Kit;Human Mxi2 ELISA Kit;Human EXIP ELISA Kit;Human PRKM14 ELISA Kit;Human PRKM15 ELISA Kit;Human RK ELISA Kit;Human p38 ELISA Kit;Human p38ALPHA ELISA Kit;Human mitogen-activated protein kinase 14 ELISA Kit;Human Csaids binding protein ELISA Kit;Human cytokine suppressive anti-inflammatory drug binding protein ELISA Kit;Human p38 MAP kinase ELISA Kit;Human p38 mitogen activated protein kinase ELISA Kit;Human p38alpha Exip ELISA Kit;
Catalog
E024703
species
Human
GeneMAPK14
SpecificityThis assay has high sensitivity and excellent specificity for detection of MAPK14. No significant cross-reactivity or interference between MAPK14 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MAPK14 is typically less than 0.56ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman MAPK14 ELISA Kit allows for the in vitro quantitative determination of MAPK14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAPK14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to APK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAPK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MAPK14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Mitogen-activated protein kinase 14 Protein information
Uniprot ID MK14_HUMAN
Uniprot AC Q8TDX0;
UniGene Hs.485233;
GeneID 1432
KEGG hsa:1432;
Human Mitogen-activated protein kinase 14 Protein SEQUENCE
SEQUENCE 360 AA; 41293 MW; 286C81D0487618B3 CRC64;
MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGLRV
AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN
DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD
LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY
NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI
SSESARNYIQ SLTQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA
QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP
PPLDQEEMES
UCSC uc003olp.4; human. [Q16539-1];

Category

Human MAPK14 ELISA Kit