Human MOTS-c ELISA Kit

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  • Catalog
    E024647
  • species
    Human
  • GeneMOTS-c
  • Standard CurveHuman MOTS-c ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MOTS-c. No significant cross-reactivity or interference between MOTS-c and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.95ng/mL.
  • Intended UseHuman MOTS-c ELISA Kit allows for the in vitro quantitative determination of MOTS-c , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MOTS-c in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to MOTS-c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MOTS-c and unlabeled MOTS-c (Standards or samples) with the pre-coated antibody specific to MOTS-c. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of MOTS-c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of MOTS-c in the sample.



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