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Human TOMM20 ELISA Kit

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  • Alternative name

    Human mitochondrial 20 kDa outer membrane protein ELISA Kit;Human outer mitochondrial membrane receptor Tom20 ELISA Kit;Human KIAA0016 ELISA Kit;Human MAS20 ELISA Kit;Human MOM19 ELISA Kit;Human TOM20 ELISA Kit;Human translocase of outer mitochondrial membrane 20 ELISA Kit;Human mitochondrial import receptor subunit TOM20 homolog ELISA Kit;Human translocase of outer mitochondrial membrane 20 homolog type II ELISA Kit;
  • Catalog
    E024635
  • species
    Human
  • GeneTOMM20
  • Standard CurveHuman TOMM20 ELISA Kit
  • Other Species Mouse Tomm20 ELISA KitBovine TOMM20 ELISA KitRat Tomm20 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TOMM-20. No significant cross-reactivity or interference between TOMM-20 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TOMM-20 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman TOMM20 ELISA Kit allows for the in vitro quantitative determination of TOMM20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This TOMM-20 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TOMM-20. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||TOMM-20 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TOMM-20 antibody and an TOMM-20-HRP conjugate. The assay sample and buffer are incubated together with TOMM-20-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TOMM-20 concentration since TOMM-20 from samples and TOMM-20-HRP conjugate compete for the anti-TOMM-20 antibody binding site. Since the number of sites is limited, as more sites are occupied by TOMM-20 from the sample, fewer sites are left to bind TOMM-20-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TOMM-20 concentration in each sample is interpolated from this standard curve.
  • Human Mitochondrial import receptor subunit TOM20 homolog Protein information
  • Uniprot ID TOM20_HUMAN
  • Uniprot AC Q15388; A8K195; Q498B3; Q6IBT4;
  • UniGene Hs.533192;
  • GeneID 9804
  • KEGG hsa:9804;
  • Human Mitochondrial import receptor subunit TOM20 homolog Protein SEQUENCE

  • SEQUENCE 145 AA; 16298 MW; 5153BD25AC5D9B3A CRC64;

    MVGRNSAIAA GVCGALFIGY CIYFDRKRRS DPNFKNRLRE RRKKQKLAKE

    RAGLSKLPDL KDAEAVQKFF LEEIQLGEEL LAQGEYEKGV DHLTNAIAVC

    GQPQQLLQVL QQTLPPPVFQ MLLTKLPTIS QRIVSAQSLA EDDVE

  • UCSC uc001hwl.4; human.;


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