SpecificityThis assay has high sensitivity and excellent specificity for detection of TOMM-20. No significant cross-reactivity or interference between TOMM-20 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TOMM-20 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman TOMM20 ELISA Kit allows for the in vitro quantitative determination of TOMM20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description specificalIntended Uses: This TOMM-20 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TOMM-20. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||TOMM-20 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TOMM-20 antibody and an TOMM-20-HRP conjugate. The assay sample and buffer are incubated together with TOMM-20-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TOMM-20 concentration since TOMM-20 from samples and TOMM-20-HRP conjugate compete for the anti-TOMM-20 antibody binding site. Since the number of sites is limited, as more sites are occupied by TOMM-20 from the sample, fewer sites are left to bind TOMM-20-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TOMM-20 concentration in each sample is interpolated from this standard curve.
Human Mitochondrial import receptor subunit TOM20 homolog Protein information
Q15388; A8K195; Q498B3; Q6IBT4;
Human Mitochondrial import receptor subunit TOM20 homolog Protein SEQUENCE