Intended UseHuman MIP-1 beta ELISA Kit allows for the in vitro quantitative determination of MIP-1 beta , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalProduct Information: MIP-1 Beta (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human MIP-1 beta in serum, plasma, urine & cell culture supernatants. Detection Range: 2.5 pg/ml - 1,000 pg/ml. 100 assays.
Background: Biobool's Human MIP-1 beta (MIP-1 beta) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-1 beta. This assay employs an antibody specific for human MIP-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIP-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha,TNF-beta, TPO, VEGF). The minimum detectable dose of MIP-1 beta is typically less than 2.5 pg/ml. Detection Range: 2.5 pg/ml - 1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- human
Application- Quantitative protein detection, establishing normal range, validation of antibody array results